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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Luchowski, R; Gryczynski, Z; Foldes-Papp, Z; Chang, A; Borejdo, J; Sarkar, P; Gryczynski, I.
Polarized fluorescent nanospheres.
Opt Express. 2010; 18(5):4289-4299
Doi: 10.1364/OE.18.004289
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- Földes-Papp Zeno
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- Abstract:
- Fluorescent beads (nanoparticles, nanospheres) are commonly used in fluorescence spectroscopy and microscopy. Due to the random distribution of dye and high dye to nanoparticle ratio, the fluorescence polarization observed from the beads is low. Therefore beads are not used for polarization study. We demonstrate that photoselective bleaching creates beads with highly polarized fluorescence. First, the beads were immobilized in a PVA polymer. Second, the beads-doped PVA film was exposed to the illumination within the dye absorption band. A progressive decrease of absorption was observed. Next, photophysical properties of photobleached and not bleached films dissolved in water were compared.
- Find related publications in this database (using NLM MeSH Indexing)
- Absorption -
- Fluorescence -
- Fluorescence Polarization -
- Nanospheres - chemistry
- Photobleaching -
- Polyvinyl Alcohol - chemistry
- Spectrometry, Fluorescence -
- Time Factors -