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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Wagner, BM; Donnarumma, F; Wintersteiger, R; Windischhofer, W; Leis, HJ.
Simultaneous quantitative determination of alpha-ketoglutaric acid and 5-hydroxymethylfurfural in human plasma by gas chromatography-mass spectrometry.
Anal Bioanal Chem. 2010; 396(7): 2629-2637.
Doi: 10.1007/s00216-010-3479-0
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- Führende Autor*innen der Med Uni Graz
- Leis Hans-Joerg
- Co-Autor*innen der Med Uni Graz
- Windischhofer Werner
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- Abstract:
- Alpha-ketoglutaric acid (alpha-KG) and 5-hydroxymethylfurfural (5-HMF) are currently under investigation as promising cancer cell damaging agents. A method for the simultaneous quantitative determination of alpha-KG and 5-HMF in human plasma was established for screening these compounds in human plasma. Plasma samples were directly treated with O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine hydrochloride to form the corresponding oximes, thus facilitating subsequent liquid-liquid extraction. After formation of the trimethylsilyl ethers, samples were analyzed by gas chromatography with electron ionization mass spectrometry. Stable isotope labeled standards were used, the preparation of (13)C(6)-5-HMF is described. Limits of quantitation were set to 0.938 microg/mL for alpha-KG and 0.156 microg/mL for 5-HMF. Inter-day accuracy was < or = 93.7% (alpha-KG) and < or = 92.8% (5-HMF). Inter-day precision was < or = 6.0% (alpha-KG) and < or = 4.6% (5-HMF). The method has been successfully applied to pharmacokinetic profiling of the compounds after intravenous application.
- Find related publications in this database (using NLM MeSH Indexing)
- Antineoplastic Agents - blood
- Blood Chemical Analysis - methods
- Complex Mixtures - analysis
- Furaldehyde - analogs and derivatives
- Gas Chromatography-Mass Spectrometry - methods
- Humans -
- Ketoglutaric Acids - blood
- Reproducibility of Results -
- Sensitivity and Specificity -
- Find related publications in this database (Keywords)
- alpha-Ketoglutaric acid
- 5-Hydroxymethylfurfural
- GC-MS