Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Navigation/Suche
- Forscher*innen
- Institutionen
- Projekte
- Publikationen
- Partner
- Geldgeber
- Ausstattung
- Knowhow
- Forschungsfelder
"Köpfe" der aktuellen Meldungen:
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kratzer, I; Bernhart, E; Wintersperger, A; Hammer, A; Waltl, S; Malle, E; Sperk, G; Wietzorrek, G; Dieplinger, H; Sattler, W.
Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier.
J Neurochem. 2009; 108(3): 707-718.
Doi: 10.1111/j.1471-4159.2008.05796.x
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Kratzer Ingrid
- Sattler Wolfgang
- Co-Autor*innen der Med Uni Graz
- Bernhart Eva Maria
- Hammer Astrid
- Malle Ernst
- Waltl Sabine Evelyn
- Wintersperger Andrea
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Alpha-tocopherol (alphaTocH), a member of the vitamin E family, is essential for normal neurological function. Despite the importance of alphaTocH transport into the CNS, transfer mechanisms across the blood-brain barrier (BBB) are not entirely clear. We here investigate whether afamin, a known alphaTocH-binding protein, contributes to alphaTocH transport across an in vitro model of the BBB consisting of primary porcine brain capillary endothelial cells (BCEC) and basolaterally cultured astrocytoma cells. Exogenously added afamin had no adverse effects on BCEC viability or barrier function and was transported across BCEC Transwell cultures. Furthermore, alphaTocH transport across polarized BCEC cultures to astrocytoma cells is facilitated by afamin, though to a lesser extent than by high-density lipoprotein-mediated transport, an essential and in vivo operating alphaTocH import pathway at the cerebrovasculature. We also demonstrate that porcine BCEC endogenously synthesize afamin. In line with these in vitro findings, afamin was detected by immunohistochemistry in porcine, human postmortem, and mouse brain, where prominent staining was observed almost exclusively in the cerebrovasculature. The demonstration of afamin mRNA expression in isolated brain capillaries suggests that afamin might be a new family member of binding/transport proteins contributing to alphaTocH homeostasis at the BBB in vivo.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Astrocytes - drug effects
- Astrocytoma - metabolism
- Biological Transport, Active -
- Blood-Brain Barrier - physiology
- Blotting, Western -
- CHO Cells -
- Capillaries - metabolism
- Carrier Proteins - biosynthesis
- Cerebrovascular Circulation - physiology
- Coculture Techniques -
- Cricetinae -
- Cricetulus -
- Electrophoresis, Polyacrylamide Gel -
- Endothelial Cells - metabolism
- Fluorescent Antibody Technique -
- Glycoproteins - biosynthesis
- Humans -
- Lipoproteins, HDL - biosynthesis
- Mice -
- Mice, Inbred C57BL -
- RNA - biosynthesis
- Recombinant Proteins - biosynthesis
- Serum Albumin - biosynthesis
- Swine -
- Tetrazolium Salts -
- Thiazoles -
- alpha-Tocopherol - metabolism
- Find related publications in this database (Keywords)
- astrocytes
- brain microvascular endothelial cells
- high-density lipoprotein
- neurovascular