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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Magnes, C; Suppan, M; Pieber, TR; Moustafa, T; Trauner, M; Haemmerle, G; Sinner, FM.
Validated comprehensive analytical method for quantification of coenzyme A activated compounds in biological tissues by online solid-phase extraction LC/MS/MS.
Anal Chem. 2008; 80(15):5736-5742
Doi: 10.1021/ac800031u
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- Führende Autor*innen der Med Uni Graz
- Sinner Frank Michael
- Co-Autor*innen der Med Uni Graz
- Moustafa Tarek
- Pieber Thomas
- Trauner Michael
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- Abstract:
- We report a robust, reliable, and comprehensive analytical method for the identification and quantification of the entire class of coenzyme A (CoA) activated substances, particularly short-, medium-, and long-chain acyl-CoAs derived from various biological tissues. This online SPE-LC/MS/MS-based method is characterized by a simple three-step sample preparation: (1) addition of buffer, organic solvents, and internal standards; (2) homogenization; and (3) centrifugation. The supernatant is injected directly into the SPE-LC/MS/MS system. Identification of CoA activated compounds is performed by accurate mass determination within the HPLC run. Method validation for short-, medium-, and long-chain acyl-CoA fatty acids revealed excellent quality. Accuracy was found to be between 87 and 107% and precision was between 0.1 and 12.8% in mouse skeletal muscle. The lower limit of quantification for all investigated compounds was well below 3.1% of estimated physiological levels in 200 mg of mouse tissue. Comparable results were obtained for mouse liver, mouse brown white adipose tissue and rat liver. For all investigated tissues, no matrix effect was observed.
- Find related publications in this database (using NLM MeSH Indexing)
- Chemistry Techniques, Analytical - methods
- Chemistry Techniques, Analytical - standards
- Chromatography, Liquid -
- Coenzyme A - metabolism
- Crown Ethers -
- Electrochemistry - methods
- Oligopeptides - analysis
- Solid Phase Extraction -
- Tandem Mass Spectrometry - methods
- Tandem Mass Spectrometry - standards