Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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"Köpfe" der aktuellen Meldungen:

Nina Höller

Nariae Baik-Schneditz

Bernhard Schwaberger

Lukas Peter Mileder

Niels Hammer

Gerhard Pichler

Roland Malli

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Hiden, U; Wadsack, C; Prutsch, N; Gauster, M; Weiss, U; Frank, HG; Schmitz, U; Fast-Hirsch, C; Hengstschlager, M; Potgens, A; Ruben, A; Knofler, M; Haslinger, P; Huppertz, B; Bilban, M; Kaufmann, P; Desoye, G.
The first trimester human trophoblast cell line ACH-3P: a novel tool to study autocrine/paracrine regulatory loops of human trophoblast subpopulations--TNF-alpha stimulates MMP15 expression.
BMC Dev Biol. 2007; 7(6): 137-137. Doi: 10.1186/1471-213X-7-137 [OPEN ACCESS]
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Führende Autor*innen der Med Uni Graz: Hiden Ursula; Wadsack Christian
Co-Autor*innen der Med Uni Graz: Desoye Gernot; Gauster Martin; Huppertz Berthold
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Abstract:: Background: The trophoblast compartment of the placenta comprises various subpopulations with distinct functions. They interact among each other by secreted signals thus forming autocrine or paracrine regulatory loops. We established a first trimester trophoblast cell line (ACH-3P) by fusion of primary human first trimester trophoblasts (week 12 of gestation) with a human choriocarcinoma cell line (AC1-1). Results: Expression of trophoblast markers (cytokeratin-7, integrins, matrix metalloproteinases), invasion abilities and transcriptome of ACH-3P closely resembled primary trophoblasts. Morphology, cytogenetics and doubling time was similar to the parental AC1-1 cells. The different subpopulations of trophoblasts e. g., villous and extravillous trophoblasts also exist in ACH-3P cells and can be immuno-separated by HLA-G surface expression. HLA-G positive ACH-3P display pseudopodia and a stronger expression of extravillous trophoblast markers. Higher expression of insulin-like growth factor II receptor and human chorionic gonadotropin represents the basis for the known autocrine stimulation of extravillous trophoblasts. Conclusion: We conclude that ACH-3P represent a tool to investigate interaction of syngeneic trophoblast subpopulations. These cells are particularly suited for studies into autocrine and paracrine regulation of various aspects of trophoblast function. As an example a novel effect of TNF-alpha on matrix metalloproteinase 15 in HLA-G positive ACH-3P and explants was found.
Find related publications in this database (using NLM MeSH Indexing): Autocrine Communication -; Cell Fusion -; Cell Line -; Cell Line, Tumor -; Cell Separation -; Choriocarcinoma -; Chorionic Gonadotropin - secretion; Female - secretion; Flow Cytometry - secretion; HLA Antigens - metabolism; Histocompatibility Antigens Class I - metabolism; Humans - metabolism; Immunohistochemistry - metabolism; Matrix Metalloproteinase 15 - biosynthesis; Neoplasm Invasiveness - biosynthesis; Oligonucleotide Array Sequence Analysis - biosynthesis; Paracrine Communication - biosynthesis; Pregnancy - biosynthesis; Pregnancy Trimester, First - biosynthesis; Reverse Transcriptase Polymerase Chain Reaction - biosynthesis; Trophoblasts - cytology; Tumor Necrosis Factor-alpha - pharmacology