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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Birner-Gruenberger, R; Hermetter, A.
Activity-based proteomics of lipolytic enzymes.
Curr Drug Discov Technol. 2007; 4(1): 1-11.
Doi: 10.2174/157016307781115458
PubMed
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- Führende Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
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- Abstract:
- Lipases play fundamental roles in biological processes since hydrolysis of triacylglycerols and cholesteryl esters is a key event in energy homeostasis of animals. Perturbations in the metabolism and the cellular retention of lipids result in common diseases such as obesity, type 2 diabetes, and atherosclerosis. The introduction of active site-directed chemical probes for enzymatic activity profiling in complex mixtures, known as activity-based proteomics, has greatly facilitated and accelerated global analysis and functional annotation of lipolytic proteins. Here we review probe design and application for discovery and discrimination of lipolytic and esterolytic enzymes. These probes are usually detected by their fluorescent or affinity tags and their protein targets are analyzed using established proteomics techniques. Moreover, microarray technologies can be applied for higher throughput screenings of enzyme or probe specificity.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Binding Sites -
- Biological Markers - chemistry
- Drug Design - chemistry
- Drug Evaluation, Preclinical - chemistry
- Enzyme Inhibitors - chemical synthesis
- Fluorescent Dyes - chemical synthesis
- Humans - chemical synthesis
- Lipase - antagonists and inhibitors
- Phosphonic Acids - chemical synthesis
- Protein Array Analysis - chemical synthesis
- Proteomics - methods
- Sterol Esterase - antagonists and inhibitors
- Structure-Activity Relationship - antagonists and inhibitors