Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Navigation/Suche
- Forscher*innen
- Institutionen
- Projekte
- Publikationen
- Partner
- Geldgeber
- Ausstattung
- Knowhow
- Forschungsfelder
"Köpfe" der aktuellen Meldungen:
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Földes-Papp, Z; Costa, JM; Demel, U; Tilz, GP; Kinjo, M; Saito, K; Kii, H; Takagi, T; Tamura, M; Thyberg, P; Birch-Hirschfeld, E.
Specifically associated PCR products probed by coincident detection of two-color cross-correlated fluorescence intensities in human gene polymorphisms of methylene tetrahydrofolate reductase at site C677T: a novel measurement approach without follow-up mathematical analysis.
Exp Mol Pathol. 2004; 76(3):212-218
Doi: 10.1016/j.yexmp.2003.12.007
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Földes-Papp Zeno
- Co-Autor*innen der Med Uni Graz
- Demel Ulrike
- Tilz Gernot
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Whole blood samples of known methylene tetrahydrofolate reductase (MTHFR) genotypes from 24 individuals were examined at site C677T. Their amplified DNA products were assessed by two-color fluorescence cross-correlation measurements and agarose gel electrophoresis/capillary gel electrophoresis. DNA subpopulations were identified which were not associated with the proper genotype by primer combinations and cycling conditions called multiplexes. We confirmed that DNA analysis by two-color fluorescence cross-correlation measurements allowed the detection of fluorescence signals specifically associated with the proper genotypes in a mixture of amplified nontarget DNA molecules without DNA sizing. The measurement approach does not require complex, follow-up mathematical analysis and is applicable to any single nucleotide polymorphisms. The simple immunogenetic model showed how the approach works to reveal specific DNA target by preventing detection of nontarget DNA. Under those experimental conditions, a new ultrasensitive, and specific method for clinical immunologists is born.
- Find related publications in this database (using NLM MeSH Indexing)
- DNA - blood
- DNA Primers - genetics
- Fluorescent Dyes - diagnostic use
- Follow-Up Studies - diagnostic use
- Genotype - diagnostic use
- Humans - diagnostic use
- Mathematics - diagnostic use
- Methylenetetrahydrofolate Reductase (NADPH2) - blood
- Polymerase Chain Reaction - blood
- Polymorphism, Single Nucleotide - blood
- Sensitivity and Specificity - blood
- Spectrometry, Fluorescence - methods
- Find related publications in this database (Keywords)
- genotype mapping
- methylene tetrahydrofolate reductase (MTHFR) deficiency at site C677T
- two-color fluorescence cross-correlation
- spectroscopy
- specific detection of amplified DNA subpopulations