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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Susani-Etzerodt, H; Schmidinger, H; Riesenhuber, G; Birner-Gruenberger, R; Hermetter, A.
A versatile library of activity-based probes for fluorescence detection and/or affinity isolation of lipolytic enzymes.
Chem Phys Lipids. 2006; 144(1): 60-68.
Doi: 10.1016/j.chemphyslip.2006.06.018
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
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- Abstract:
- This work describes the synthesis of a library of fluorescent and/or biotinylated alkylphosphonate inhibitors being reactive towards serine hydrolases, especially lipases and esterases. Fluorescent inhibitors can be used for sensitive and rapid detection of active proteins by gel electrophoresis. Biotinylated inhibitors are applicable for the enrichment and isolation of active enzymes. Functionality as well as the different detection methods of the synthesized inhibitors were successfully tested with an enzyme preparation, namely cholesterol esterase from porcine pancreas (ppCE). Moreover, a biotinylated inhibitor was employed to enrich ppCE on avidin beads. Hence, our set of phosphonate inhibitors can be used for the detection and/or isolation of active serine hydrolases.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Avidin - chemistry
- Cholesterol Esterase - chemistry
- Enzyme Inhibitors - chemical synthesis
- Esterases - chemical synthesis
- Fluorescence - chemical synthesis
- Indicators and Reagents - chemical synthesis
- Lipase - chemical synthesis
- Microscopy, Fluorescence - chemical synthesis
- Molecular Structure - chemical synthesis
- Organophosphorus Compounds - chemical synthesis
- Pancreas - chemistry
- Swine - chemistry
- Find related publications in this database (Keywords)
- activity-based enzyme probes
- alkylphosphonic acid ester
- biotin-labeled inhibitors
- cholesterol esterase
- fluorescent lipids
- serine hydrolases