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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Schmidinger, H; Susani-Etzerodt, H; Birner-Gruenberger, R; Hermetter, A.
Inhibitor and protein microarrays for activity-based recognition of lipolytic enzymes.
Chembiochem. 2006; 7(3): 527-534. Doi: 10.1002/cbic.200500276
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Co-Autor*innen der Med Uni Graz
Birner-Grünberger Ruth
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Abstract:
Protein and small-molecule microarrays are useful tools for high-throughput analysis of DNA-protein, protein-protein, and protein-small molecule interactions. Here we report on novel microarrays for activity screening of lipases and esterases based on phosphonic acid ester inhibitors. These compounds are activity recognition probes (ARPs) and bind to active serine hydrolases in a stoichiometric and irreversible manner. Protein microarrays were generated by spotting six different lipolytic enzymes onto hydrogel-coated glass slides. The activity of immobilized enzymes was determined after treatment with fluorescently labeled ARPs. Alternatively, biotinylated ARPs were bound to streptavidin slides in order to identify their affinity for enzymes in solution. Both systems, the protein- and ARP microarrays proved to be useful and versatile tools for the rapid identification and characterization of novel and known lipolytic enzymes.
Find related publications in this database (using NLM MeSH Indexing)
Enzyme Inhibitors - pharmacology
Enzymes - metabolism
Lipolysis - metabolism
Protein Array Analysis - metabolism

Find related publications in this database (Keywords)
fluorescent probes
hydrolases
inhibitors
protein microarrays
small-molecule microarrays
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