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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Koidl, J; Hödl, H; Schmid, MG; Konrad, M; Petschauer, S; Kostner, GM; Gübitz, G.
Chiral separation of T3 enantiomers using stereoselective antibodies as a selector in micro-HPLC.
J BIOCHEM BIOPHYS METH. 2006; 69(1-2): 33-42.
Doi: 10.1016/j.jbbm.2006.03.015
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- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
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- Abstract:
- This work deals with the application of stereoselective antibodies against L-T3 as a tailor-made chiral selector in micro-HPLC. The separations were performed in microbore columns using commercially available anti-L-T3 antibodies chemically bonded to 5 microm silica gel. The enantiomers of T3 were baseline separated under mild continuous isocratic elution conditions using 10 mM phosphate buffer, pH 7.4. The D-enantiomer eluted with the void volume, while the L-enantiomer was retained by the antibody phase and eluted second. An indirect competitive and non-competitive enzyme linked immunosorbent assay (ELISA) was used for testing the stereoselectivity of anti-L-T3 antibodies.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibody Specificity -
- Chromatography, Affinity -
- Chromatography, High Pressure Liquid -
- Enzyme-Linked Immunosorbent Assay -
- Microchemistry -
- Stereoisomerism -
- Triiodothyronine - chemistry
- Find related publications in this database (Keywords)
- micro-HPLC
- thyroid hormones
- stereoselective antibodies
- enantiomer separation
- enzyme-linked immunosorbent assay