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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Popov, Y; Patsenker, E; Fickert, P; Trauner, M; Schuppan, D.
Mdr2 (Abcb4)-/- mice spontaneously develop severe biliary fibrosis via massive dysregulation of pro- and antifibrogenic genes.
J HEPATOL. 2005; 43(6): 1045-1054. Doi: 10.1016/j.jhep.2005.06.025
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Co-Autor*innen der Med Uni Graz
Fickert Peter
Trauner Michael
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Abstract:
Background/Aims: Mdr2 (Abcb4)-/- mice develop hepatic lesions resembling primary sclerosing cholangitis. Our aim was to characterize the evolution of fibrosis in Mdr2-/- mice. Methods: Mdr2-/-mice and their wild-type littermates were sacrificed at 2,4 and 8 weeks after birth. Hepatic collagen was determined biochemically. Fibrosis related transcript levels were quantified from livers by real-time RT-PCR, and NIMP activities determined by substrate assays. Liver histology was assessed by connective tissue staining and immunohistochemistry for alpha-smooth muscle actin (alpha-SMA). Results: Mdr2-/- mice demonstrated a time-dependent increase of relative and total hepatic collagen (fivefold at 8 weeks, compared to wildtype controls), and maximal alpha-SMA immunoreactivity at 4 weeks. Compared to wildtype controls profibrogenic mRNA levels for procollagen alpha 1(I), TGF beta 1, TGF beta 2, MMP-2 and -13, TIMP-1, PDGF beta receptor, and PAI-1 were upregulated up to 27-fold. Most transcripts peaked at 4 weeks, but procollagen alpha 1(l) mRNA increased steadily, TIMP-1 mRNA was constantly elevated (20-fold), MMP-13 mRNA was suppressed and interstitial collagenase and gelatinase activities were downregulated. Conclusions: Mdr2-/- mice spontaneously progress to severe biliary fibrosis. This is due to a characteristic temporal pattern of upregulated profibrogenic and downregulated fibrolytic genes and activities. These mice are an attractive model to test potential antifibrotics for the treatment of (biliary) liver fibrosis. (c) 2005 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
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Animals -
Bile Ducts, Intrahepatic - pathology
Disease Models, Animal -
Liver Cirrhosis - genetics
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Mice, Knockout -
P-Glycoproteins - genetics

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animal model
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collagen
liver fibrosis
knockout mice
PAI-1
PDGF receptor
procollagen
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TGF-beta
TIMP-1
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