Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Navigation/Suche
- Forscher*innen
- Institutionen
- Projekte
- Publikationen
- Partner
- Geldgeber
- Ausstattung
- Knowhow
- Forschungsfelder
"Köpfe" der aktuellen Meldungen:
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Birner-Gruenberger, R; Susani-Etzerodt, H; Waldhuber, M; Riesenhuber, G; Schmidinger, H; Rechberger, G; Kollroser, M; Strauss, JG; Lass, A; Zimmermann, R; Haemmerle, G; Zechner, R; Hermetter, A.
The lipolytic proteome of mouse adipose tissue.
MOL CELL PROTEOMICS. 2005; 4(11): 1710-1717.
Doi: 10.1074/mcp.M500062-MCP200
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
- Co-Autor*innen der Med Uni Graz
- Kollroser Manfred
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Hydrolysis of triacylglycerols and cholesteryl esters is a key event in energy homeostasis of animals. However, many lipolytic activities still await their molecular identification. Here we report on a novel tool for concomitant analysis of lipases in complex proteomes. Fluorescent activity tags mimicking lipid substrates were used to label the proteome of mouse adipose tissue. Analysis by two-dimensional gel electrophoresis and LC-MS/MS led to the identification of all known intracellular lipases as well as a number of novel candidates. One of them was recently shown to be involved in triacylglycerol mobilization in adipocytes and therefore named adipose triglyceride lipase. Functional characterization of expressed enzymes demonstrated that lipolytic and esterolytic activities could be well discriminated. Thus our results show the first map of the lipolytic proteome of mouse adipose tissue and demonstrate the general applicability of our method for rapid profiling and identification of lipolytic activities in complex biological samples.
- Find related publications in this database (using NLM MeSH Indexing)
- 4-Chloro-7-nitrobenzofurazan - analogs and derivatives
- Adipose Tissue - metabolism
- Animals - metabolism
- Cholesterol Esterase - deficiency
- Lipase - metabolism
- Lipolysis - drug effects
- Mice - drug effects
- Mice, Knockout - drug effects
- Proteome - metabolism