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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Rohde, E; Malischnik, C; Thaler, D; Maierhofer, T; Linkesch, W; Lanzer, G; Guelly, C; Strunk, D.
Blood monocytes mimic endothelial progenitor cells.
Stem Cells. 2006; 24(2): 357-367.
Doi: 10.1634/stemcells.2005-0072
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- Führende Autor*innen der Med Uni Graz
- Rohde Eva
- Strunk Dirk
- Co-Autor*innen der Med Uni Graz
- Gülly Christian
- Lanzer Gerhard
- Linkesch Werner
- Schnur Theresa
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- Abstract:
- The generation of endothelial progenitor cells (EPCs) from blood monocytes has been propagated as a novel approach in the diagnosis and treatment of cardiovascular diseases. Low-density lipoprotein (LDL) uptake and lectin binding together with endothelial marker expression are commonly used to define these EPCs. Considerable controversy exists regarding their nature, in particular, because myelomonocytic cells share several properties with endothelial cells (ECs). This study was performed to elucidate whether the commonly used endothelial marker determination is sufficient to distinguish supposed EPCs from monocytes. We measured endothelial, hematopoietic, and progenitor cell marker expression of monocytes before and after angiogenic culture by fluorescence microscopy, flow cytometry, and real-time reverse transcription-polymerase chain reaction. The function of primary monocytes and monocyte-derived supposed EPCs was investigated during vascular network formation and EC colony-forming unit (CFU-EC) development. Monocytes cultured for 4 to 6 days under angiogenic conditions lost CD14/CD45 and displayed a commonly accepted EPC phenotype, including LDL uptake and lectin binding, CD31/CD105/CD144 reactivity, and formation of cord-like structures. Strikingly, primary monocytes already expressed most tested endothelial genes and proteins at even higher levels than their supposed EPC progeny. Neither fresh nor cultured monocytes formed vascular networks, but CFU-EC formation was strictly dependent on monocyte presence. LDL uptake, lectin binding, and CD31/CD105/CD144 expression are inherent features of monocytes, making them phenotypically indistinguishable from putative EPCs. Consequently, monocytes and their progeny can phenotypically mimic EPCs in various experimental models.
- Find related publications in this database (using NLM MeSH Indexing)
- Biological Markers - analysis
- Cell Differentiation - analysis
- Cells, Cultured - analysis
- Cholesterol, LDL - blood
- Endothelial Cells - metabolism
- Erythroid Progenitor Cells - physiology
- Flow Cytometry - physiology
- HL-60 Cells - physiology
- Humans - physiology
- Lectins - blood
- Male - blood
- Microscopy, Fluorescence - blood
- Monocytes - metabolism
- Neovascularization, Physiologic - metabolism
- Polymerase Chain Reaction - metabolism
- Reverse Transcriptase Polymerase Chain Reaction - metabolism
- Stem Cells - physiology
- Up-Regulation - physiology
- Find related publications in this database (Keywords)
- monocytes
- endothelial progenitor cells
- endothelial cells
- circulating angiogenic cells
- circulating endothelial progenitors
- circulating endothelial cells