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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Horstmann, H; Michel, NA; Sheng, X; Hansen, S; Lindau, A; Pfeil, K; Fernández, MC; Marchini, T; Winkels, H; Mitre, LS; Abogunloko, T; Li, X; Mwinyella, TB; Gissler, MC; Bugger, H; Heidt, T; Buscher, K; Hilgendorf, I; Stachon, P; Piepenburg, S; Verheyen, N; Rathner, T; Gerhardt, T; Siegel, PM; Oswald, WK; Cohnert, T; Zernecke, A; Madl, J; Kohl, P; Foks, AC; von, Zur, Muehlen, C; Westermann, D; Zirlik, A; Wolf, D.
Cross-species single-cell RNA sequencing reveals divergent phenotypes and activation states of adaptive immunity in human carotid and experimental murine atherosclerosis.
Cardiovasc Res. 2024; 120(14): 1713-1726.
Doi: 10.1093/cvr/cvae154
[OPEN ACCESS]
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PubMed
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- Leading authors Med Uni Graz
- Anto Michel Nathaly
- Zirlik Andreas
- Co-authors Med Uni Graz
- Bugger Heiko Matthias
- Cohnert Tina Ulrike
- Oswald Wolfgang Kurt
- Pfeil Katharina
- Rathner Thomas
- Verheyen Nicolas Dominik
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- Abstract:
- AIMS: The distinct functions of immune cells in atherosclerosis have been mostly defined by pre-clinical mouse studies. Contrastingly, the immune cell composition of human atherosclerotic plaques and their contribution to disease progression are only poorly understood. It remains uncertain whether genetic animal models allow for valuable translational approaches. METHODS AND RESULTS: Single-cell RNA-sequencing (scRNA-seq) was performed to define the immune cell landscape in human carotid atherosclerotic plaques. The human immune cell repertoire demonstrated an unexpectedly high heterogeneity and was dominated by cells of the T-cell lineage, a finding confirmed by immunohistochemistry. Bioinformatical integration with 7 mouse scRNA-seq data sets from adventitial and atherosclerotic vascular tissue revealed a total of 51 identities of cell types and differentiation states, of which some were only poorly conserved between species and exclusively found in humans. Locations, frequencies, and transcriptional programmes of immune cells in mouse models did not resemble the immune cell landscape in human carotid atherosclerosis. In contrast to standard mouse models of atherosclerosis, human plaque leucocytes were dominated by several T-cell phenotypes with transcriptional hallmarks of T-cell activation and memory formation, T-cell receptor, and pro-inflammatory signalling. Only mice at the age of 22 months partially resembled the activated T-cell phenotype. In a validation cohort of 43 patients undergoing carotid endarterectomy, the abundance of activated immune cell subsets in the plaque defined by multi-colour flow cytometry associated with the extent of clinical atherosclerosis. CONCLUSION: Integrative scRNA-seq reveals a substantial difference in the immune cell composition of murine and human carotid atherosclerosis-a finding that questions the translational value of standard mouse models for adaptive immune cell studies. Clinical associations suggest a specific role for T-cell driven (auto-)immunity in human plaque formation and instability.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Humans - administration & dosage
- Single-Cell Analysis - administration & dosage
- Disease Models, Animal - administration & dosage
- Adaptive Immunity - genetics
- Phenotype - administration & dosage
- Carotid Artery Diseases - immunology, genetics, pathology, metabolism
- Plaque, Atherosclerotic - administration & dosage
- RNA-Seq - administration & dosage
- Male - administration & dosage
- Mice, Inbred C57BL - administration & dosage
- Species Specificity - administration & dosage
- Transcriptome - administration & dosage
- T-Lymphocytes - immunology, metabolism
- Female - administration & dosage
- Mice, Knockout, ApoE - administration & dosage
- Carotid Arteries - immunology, pathology, metabolism
- Lymphocyte Activation - administration & dosage
- Middle Aged - administration & dosage
- Aged - administration & dosage
- Find related publications in this database (Keywords)
- Atherosclerosis
- Leucocytes
- ScRNA-seq
- Transcriptome
- Prediction
- Immunity