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SHR Neuro Cancer Cardio Lipid Metab Microb
Patra, V; Woltsche, N; Cerpes, U; Bokanovic, D; Repelnig, M; Joshi, A; Perchthaler, I; Fischl, M; Vocanson, M; Bordag, N; Durdevic, M; Woltsche, J; Quehenberger, F; Legat, F; Wedrich, A; Horwath-Winter, J; Wolf, P.
Persistent Neutrophil Infiltration and Unique Ocular Surface Microbiome Typify Dupilumab-Associated Conjunctivitis in Patients with Atopic Dermatitis.
Ophthalmol Sci. 2024; 4(1): 100340
Doi: 10.1016/j.xops.2023.100340
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- Leading authors Med Uni Graz
- Patra Vijaykumar
- Wolf Peter
- Woltsche Nora
- Co-authors Med Uni Graz
- Bokanovic Danijela
- Bordag Natalie
- Cerpes Urban
- Durdevic Marija
- Horwath-Winter Jutta
- Joshi Aaroh Anand
- Legat Franz
- Perchthaler Isabella
- Quehenberger Franz
- Repelnig Maria-Lisa
- Wedrich Andreas
- Woltsche Johannes Nikolaus
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- Abstract:
- OBJECTIVE: To elucidate the pathogenesis of dupilumab (Dupixent®)-associated ocular surface disease (DAOSD). DESIGN: Prospective single-center cohort study. SUBJECTS: Twenty patients with moderate-to-severe atopic dermatitis (AD) who received dupilumab and 10 age- and sex-matched healthy controls were enrolled in the study. METHODS: The study cohort underwent a thorough slit-lamp and entire-body dermatologic examination. Conjunctival swabs and smears were collected at baseline, 4 and 16 weeks after treatment initiation, and during the conjunctivitis episode. To analyse the ocular surface microbiome, 16S ribosomal RNA sequencing was performed, smears were hematoxylin and eosin stained, and serum cytokines were measured by using a multiplex immunobead assay. MAIN OUTCOME MEASURES: Composition of ocular surface microbiome and cellular component as well as serum cytokine levels. RESULTS: Six of the 20 patients with AD developed DAOSD after dupilumab initiation; these patients responded after a delay to treatment as quantified by Eczema Area and Severity Index and Investigator's Global Assessment score. Conjunctival smears showed massive neutrophilic infiltration and serum analysis revealed increased systemic levels of neutrophil-priming proinflammatory cytokines, in particular interleukin-1β and tumor necrosis factor α, in patients with DAOSD compared with those without it. The ocular surface microbiome of patients with DAOSD was characterized by a diverse and persistent microbial colonization, particularly by Acetobacter aceti. In contrast, microbial diversity decreased in patients with AD without DAOSD after the initiation of dupilumab treatment, especially the abundance of Staphylococcus aureus. In vitro experiments substantiated the potential role of the microbiome, showing increased growth of A. aceti and decreased growth of S. aureus in presence of dupilumab. CONCLUSIONS: Persistent neutrophilic infiltration and a unique microbial landscape on the ocular surface associated with elevated levels of systemic proinflammatory cytokines typify DAOSD. FINANCIAL DISCLOSURES: Proprietary or commercial disclosure may be found after the references.
- Find related publications in this database (Keywords)
- Dupilumab
- Atopic dermatitis
- Microbiome
- Cytokines
- Neutrophils