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"Faces" of the day:

Katharina Jandl

Nina Höller

Nariae Baik-Schneditz

Ellen Heitzer

Marianne Brodmann

Ewald Kolesnik

Bernhard Schwaberger

Maximilian Seles

Gabor Toth-Gayor

Lukas Peter Mileder

Niels Hammer

Christian Josef Hill

Christian Viertler

Philipp Klaritsch

Daniel Scherr

Harald Köfeler

Gerhard Pichler

Roland Malli

Michael Fuchsjäger

Gernot Plank

Peter Fickert

Richard Zigeuner

Peter Holzer

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Krolitzki, E; Steck, S; Nazifi, A; Abt, M; Schwaminger, SP; Berensmeier, S.
How to design a low-cost pilot scale magnetic bioseparation process for protein separation from complex mixtures using in-line process analytics
SEP PURIF TECHNOL. 2023; 323: 124429 Doi: 10.1016/j.seppur.2023.124429
Web of Science FullText FullText_MUG

 

Leading authors Med Uni Graz

Schwaminger Sebastian

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Abstract:

Magnetic separation is a powerful tool for the integrated capture and purification of biomolecules and cells directly from complex feed media without the need for prior clarification and without the risk of equipment blockage. In this study, we equipped a commercially available rotor-stator high-gradient magnetic separator (RSHGMS) with in-line analytics of hygienic design (absorbance, conductivity, pH, and flow) and used it for a systematic characterization of the equipment. By combining particle agglomeration and separation studies with residence time analysis, magnetic filter capacity, and particle retention experiments for bare iron oxide nanoparticles (BIONs), we developed a generic RS-HGMS operation protocol optimized for minimal particle carryover and efficient buffer exchange. To demonstrate the versatility of our approach, we customized this operation protocol for the challenging task of lactoferrin separation from crude acid whey, which serves as an illustrative example in this paper. The modifications involved simple adjustments of pump speed, step duration, and cycle repetition without altering the fundamental principles of our generic approach. In this proof-of-concept, a BIONs carry-over of less than 0.037 g L-1 was achieved in the elution fractions, and the in-line sensors enabled continuous monitoring and documentation of the process, providing valuable data for process optimization and validation. This paper demonstrates how a magnetic separation process can be effortlessly tailored for challenging bioseparation tasks, while emphasizing the importance of implementing continuous in-line process monitoring and data logging. We believe this is an essential step toward the wider adoption of this promising technique in the industry.

Find related publications in this database (Keywords)

Magnetic separation

Bioseparation

Protein purification

Capture

Iron oxide nanoparticles

Lactoferrin

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