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SHR Neuro Cancer Cardio Lipid Metab Microb
Dichtl, K; Osterman, A; Forster, J; Jakob, L; Suerbaum, S; Flaig, MJ; Schubert, S; Wagener, J.
A retrospective evaluation of the Euroarray STI-11 multiplex system for the detection of eight STI causing agents.
Sci Rep. 2023; 13(1): 11382
Doi: 10.1038/s41598-023-38121-w
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- Leading authors Med Uni Graz
- Dichtl Karl
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- Abstract:
- With an incidence of more than > 1,000,000/day, sexually transmitted diseases remain a major challenge for health care systems worldwide. To reduce disease burden, complications, and spread, rapid diagnosis permitting early therapy is pivotal. The range of pathogens is wide and co-infections are common. This complicates pre-analytics, which are based on different laboratory techniques with potentially long turnaround times, e.g., cultivation and multistep serologies. Multiplex PCR provides the opportunity to overcome these limitations. In this study, we evaluated a novel assay, the Euroarray STI-11 microarray (EA; Euroimmun Medizinische Labordiagnostika), for the detection of eight obligate or facultative pathogens. Three-hundred-thirteen clinical specimens, which had been tested and pre-characterized for STI causing agents as part of routine diagnostics, were used as cases and controls in this retrospective study. The EA detected 34/44 Chlamydia trachomatis, 48/50 HSV-1, 50/50 HSV-2, 48/48 Mycoplasma hominis, 45/47 Neisseria gonorrhoeae, 9/11 Treponema pallidum, 46/46 Ureaplasma parvum, and 49/49 Ureaplasma urealyticum infections, respectively. 293 samples were EA positive, with polymicrobial infections (positive for two to six microbial or viral agents) detected in 130/293 cases. Specificities were 100% in the respective control groups (n = 18-48 depending on targeted pathogen) except for N. gonorrhoeae (25/26) and U. urealyticum (44/45). The broad spectrum of obligate and facultative pathogens targeted by the EA makes it a valuable tool in the setting of STI diagnostics and surveillance. The test has the potential to diagnose diseases neglected or overlooked in routine clinical practice. Besides a low sensitivity for C. trachomatis, the EA demonstrated high performance for all analyzed parameters. Further studies are warranted in order to capture a larger variety of the tested pathogens.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Retrospective Studies - administration & dosage
- Sexually Transmitted Diseases - diagnosis, epidemiology
- Chlamydia trachomatis - administration & dosage
- Ureaplasma - administration & dosage
- Neisseria gonorrhoeae - administration & dosage