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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Burgstaller, S; Wagner, TR; Bischof, H; Bueckle, S; Padamsey, A; Frecot, D; Kaiser, PD; Skrabak, D; Malli, R; Lukowski, R; Rothbauer, U.
Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensors.
iScience. 2022; 25(9): 104907
Doi: 10.1016/j.isci.2022.104907
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Burgstaller Sandra
- Co-Autor*innen der Med Uni Graz
- Bischof Helmut
- Malli Roland
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- Abstract:
- Ion and analyte changes in the tumor microenvironment (TME) alter the metabolic activity of cancer cells, promote tumor cell growth, and impair anti-tumor immunity. Consequently, accurate determination and visualization of extracellular changes of analytes in real time is desired. In this study, we genetically combined FRET-based biosensors with nanobodies (Nbs) to specifically visualize and monitor extracellular changes in K+, pH, and glucose on cell surfaces. We demonstrated that these Nb-fused biosensors quantitatively visualized K+ alterations on cancer and non-cancer cell lines and primary neurons. By implementing a HER2-specific Nb, we generated functional K+ and pH sensors, which specifically stained HER2-positive breast cancer cells. Based on the successful development of several Nb-fused biosensor combinations, we anticipate that this approach can be readily extended to other biosensors and will open new opportunities for the study of extracellular analytes in advanced experimental settings.