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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Marksteiner, J; Ebner, J; Salzer, I; Lilliu, E; Hackl, B; Todt, H; Kubista, H; Hallstrom, S; Koenig, X; Hilber, K.
Evidence for a Physiological Role of T-Type Ca Channels in Ventricular Cardiomyocytes of Adult Mice
MEMBRANES-BASEL. 2022; 12(6): 566 Doi: 10.3390/membranes12060566 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Co-Autor*innen der Med Uni Graz
Hallström Seth
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Abstract:
T-type Ca channels are strongly expressed and important in the developing heart. In the adult heart, these channels play a significant role in pacemaker tissues, but there is uncertainty about their presence and physiological relevance in the working myocardium. Here, we show that the T-type Ca channel isoforms Cav3.1 and Cav3.2 are expressed at a protein level in ventricular cardiomyocytes from healthy adult C57/BL6 mice. Myocytes isolated from adult wild-type and Cav3.2 KO mice showed considerable whole cell T-type Ca currents under beta-adrenergic stimulation with isoprenaline. We further show that the detectability of basal T-type Ca currents in murine wild-type cardiomyocytes depends on the applied experimental conditions. Together, these findings reveal the presence of functional T-type Ca channels in the membrane of ventricular myocytes. In addition, electrically evoked Ca release from the sarcoplasmic reticulum was significantly impaired in Cav3.2 KO compared to wild-type cardiomyocytes. Our work implies a physiological role of T-type Ca channels in the healthy adult murine ventricular working myocardium.

Find related publications in this database (Keywords)
adult murine heart
intracellular Ca release
T-type Ca channels
whole cell patch clamp
working myocardium
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