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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kienesberger, B; Obermüller, B; Singer, G; Arneitz, C; Gasparella, P; Klymiuk, I; Horvath, A; Stadlbauer, V; Magnes, C; Zügner, E; López-García, P; Trajanoski, S; Miekisch, W; Fuchs, P; Till, H; Castellani, C.
Insights into the Composition of a Co-Culture of 10 Probiotic Strains (OMNi BiOTiC® AAD10) and Effects of Its Postbiotic Culture Supernatant.
Nutrients. 2022; 14(6):
Doi: 10.3390/nu14061194
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Kienesberger Bernhard
- Obermüller Beate
- Co-authors Med Uni Graz
- Arneitz Christoph
- Castellani Christoph
- Gasparella Paolo
- Horvath Angela
- Klymiuk Ingeborg
- Lopez Garcia Pablo
- Singer Georg
- Stadlbauer-Köllner Vanessa
- Till Holger
- Trajanoski Slave
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- Abstract:
- BACKGROUND: We aimed to gain insights in a co-culture of 10 bacteria and their postbiotic supernatant. METHODS: Abundances and gene expression were monitored by shotgun analysis. The supernatant was characterized by liquid chromatography mass spectroscopy (LC-MS) and gas chromatography mass spectroscopy (GC-MS). Supernatant was harvested after 48 h (S48) and 196 h (S196). Susceptibility testing included nine bacteria and C. albicans. Bagg albino (BALBc) mice were fed with supernatant or culture medium. Fecal samples were obtained for 16S analysis. RESULTS: A time-dependent decrease of the relative abundances and gene expression of L. salivarius, L. paracasei, E. faecium and B. longum/lactis and an increase of L. plantarum were observed. Substances in LC-MS were predominantly allocated to groups amino acids/peptides/metabolites and nucleotides/metabolites, relating to gene expression. Fumaric, panthotenic, 9,3-methyl-2-oxovaleric, malic and aspartic acid, cytidine monophosphate, orotidine, phosphoserine, creatine, tryptophan correlated to culture time. Supernatant had no effect against anaerobic bacteria. S48 was reactive against S. epidermidis, L. monocytogenes, P. aeruginosae, E. faecium and C. albicans. S196 against S. epidermidis and Str. agalactiae. In vivo S48/S196 had no effect on alpha/beta diversity. Linear discriminant analysis effect size (LEfSe) and analysis of composition of microbiomes (ANCOM) revealed an increase of Anaeroplasma and Faecalibacterium prausnitzii. CONCLUSIONS: The postbiotic supernatant had positive antibacterial and antifungal effects in vitro and promoted the growth of distinct bacteria in vivo.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Anti-Bacterial Agents - pharmacology
- Bacteria - genetics
- Candida albicans - administration & dosage
- Coculture Techniques - administration & dosage
- Mice - administration & dosage
- Probiotics - pharmacology
- Find related publications in this database (Keywords)
- postbiotic
- microbiome
- volatile organic compound
- shotgun keyword
- susceptibility
- culture