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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Goeritzer, M; Schlager, S; Kuentzel, KB; Vujić, N; Korbelius, M; Rainer, S; Kolb, D; Mussbacher, M; Salzmann, M; Schrottmaier, WC; Assinger, A; Schlagenhauf, A; Madreiter-Sokolowski, CT; Blass, S; Eichmann, TO; Graier, WF; Kratky, D.
Adipose Triglyceride Lipase Deficiency Attenuates In Vitro Thrombus Formation without Affecting Platelet Activation and Bleeding In Vivo.
Cells. 2022; 11(5): Doi: 10.3390/cells11050850 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Führende Autor*innen der Med Uni Graz
Göritzer Madeleine
Kratky Dagmar
Co-Autor*innen der Med Uni Graz
Blass Sandra
Eichmann Thomas
Graier Wolfgang
Kolb Dagmar
Korbelius Melanie
Küntzel Katharina Barbara
Madreiter-Sokolowski Corina
Rainer Silvia
Schlagenhauf Axel
Schlager Stefanie
Vujic Nemanja
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Abstract:
According to genome-wide RNA sequencing data from human and mouse platelets, adipose triglyceride lipase (ATGL), the main lipase catalyzing triglyceride (TG) hydrolysis in cytosolic lipid droplets (LD) at neutral pH, is expressed in platelets. Currently, it is elusive to whether common lipolytic enzymes are involved in the degradation of TG in platelets. Since the consequences of ATGL deficiency in platelets are unknown, we used whole-body and platelet-specific (plat)Atgl-deficient (-/-) mice to investigate the loss of ATGL on platelet function. Our results showed that platelets accumulate only a few LD due to lack of ATGL. Stimulation with platelet-activating agonists resulted in comparable platelet activation in Atgl-/-, platAtgl-/-, and wild-type mice. Measurement of mitochondrial respiration revealed a decreased oxygen consumption rate in platelets from Atgl-/- but not from platAtgl-/- mice. Of note, global loss of ATGL was associated with an anti-thrombogenic phenotype, which was evident by reduced thrombus formation in collagen-coated channels in vitro despite unchanged bleeding and occlusion times in vivo. We conclude that genetic deletion of ATGL affects collagen-induced thrombosis without pathological bleeding and platelet activation.
Find related publications in this database (using NLM MeSH Indexing)
Acyltransferases - metabolism
Animals - administration & dosage
Lipase - metabolism
Mice - administration & dosage
Mice, Knockout - administration & dosage
Platelet Activation - administration & dosage
Thrombosis - administration & dosage
Triglycerides - metabolism

Find related publications in this database (Keywords)
platelets
adipose triglyceride lipase
thrombosis
mitochondrial respiration
intravital microscopy
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