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SHR Neuro Cancer Cardio Lipid Metab Microb
Narimisa, N; Sadeghi, Kalani, B; Mohammadzadeh, R; Masjedian, Jazi, F.
Combination of Antibiotics-Nisin Reduces the Formation of Persister Cell in Listeria monocytogenes.
Microb Drug Resist. 2021; 27(2): 137-144.
Doi: 10.1089/mdr.2020.0019
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Mohammadzadeh Rokhsareh
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- Abstract:
- Persister cells are a subpopulation of bacteria with the ability of survival when exposed to lethal doses of antibiotics, and are responsible for antibiotic therapy failure and infection recurrences. In this study, we investigated persister cell formation and the role of nisin in combination with antibiotics in reducing persistence in Listeria monocytogenes. We also examined the expression of toxin-antitoxin (TA) systems in persister cells of L. monocytogenes to gain a better understanding of the effect of TA systems on persister cell formation. To induce persistence, L. monocytogenes were exposed to high doses of different antibiotics over a period of 24 hr, and the expression levels of TA system was genes were measured 5 hr after the addition of antibiotics by the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) method. To investigate the effect of nisin, L. monocytogenes was exposed to a combination of nisin and antibiotics. According to our results, L. monocytogenes was highly capable of persister cell formation, and the combination of nisin and antibiotics resulted in reduced persistence. qRT-PCR results showed a significant increase in GNAT/RHH expression among the studied systems. Overall, our results demonstrated the potential of the combination of nisin and antibiotics in reducing persister cell formation, and emphasized the role of the GNAT/RHH system in bacterial persistence.
- Find related publications in this database (using NLM MeSH Indexing)
- Anti-Bacterial Agents - pharmacology
- Gene Expression Regulation, Bacterial - drug effects
- Listeria monocytogenes - drug effects
- Nisin - pharmacology
- Polymerase Chain Reaction - methods
- Toxin-Antitoxin Systems - drug effects
- Find related publications in this database (Keywords)
- Listeria monocytogenes
- nisin
- persister cells
- TA system
- real-time PCR