Medizinische Universität Graz - Research portal
Navigation/Search
- Researchers
- Institutions
- Projects
- Publications
- Partners
- Sponsors
- Equipment
- Knowhow
- Fields of Research
"Faces" of the day:
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Cisse, A; Schachner-Nedherer, AL; Appel, M; Beck, C; Ollivier, J; Leitinger, G; Prassl, R; Kornmueller, K; Peters, J.
Dynamics of Apolipoprotein B-100 in Interaction with Detergent Probed by Incoherent Neutron Scattering.
J Phys Chem Lett. 2021; 12(51):12402-12410
Doi: 10.1021/acs.jpclett.1c03141
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Kornmüller Karin
- Co-authors Med Uni Graz
- Leitinger Gerd
- Prassl Ruth
- Schachner-Nedherer Anna-Laurence
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Apolipoprotein B-100 (apo B-100) is the protein moiety of both low- and very-low-density lipoproteins, whose role is crucial to cholesterol and triglyceride transport. Aiming at the molecular dynamics' details of apo B-100, scarcely studied, we performed elastic and quasi-elastic incoherent neutron scattering (EINS, QENS) experiments combining different instruments and time scales. Similar to classical membrane proteins, the solubilization results in remaining detergent, here Nonidet P-40 (NP40). Therefore, we propose a framework for QENS studies of protein-detergent complexes, with the introduction of a combined model, including the experimental apo B-100/NP40 ratio. Relying on the simultaneous analysis of all QENS amplitudes, this approach is sensitive enough to separate both contributions. Its application identified two points: (i) apo B-100 slow dynamics and (ii) the acceleration of NP40 dynamics in the presence of apo B-100. Direct translation of the exposed methodology now makes the investigation of more membrane proteins by neutron spectroscopy achievable.
- Find related publications in this database (using NLM MeSH Indexing)
- Apolipoprotein B-100 - chemistry
- Detergents - chemistry
- Humans - administration & dosage
- Molecular Dynamics Simulation - administration & dosage
- Neutrons - administration & dosage
- Scattering, Small Angle - administration & dosage