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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Smolka, C; Schlösser, D; Koentges, C; Tarkhnishvili, A; Gorka, O; Pfeifer, D; Bemtgen, X; Asmussen, A; Groß, O; Diehl, P; Moser, M; Bode, C; Bugger, H; Grundmann, S; Pankratz, F.
Cardiomyocyte-specific miR-100 overexpression preserves heart function under pressure overload in mice and diminishes fatty acid uptake as well as ROS production by direct suppression of Nox4 and CD36.
FASEB J. 2021; 35(11):e21956
Doi: 10.1096/fj.202100829RR
Web of Science
PubMed
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FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Bugger Heiko Matthias
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- Abstract:
- MicroRNAs are key regulators of the cardiac response to injury. MiR-100 has recently been suggested to be involved in different forms of heart failure, but functional studies are lacking. In the present study, we examined the impact of transgenic miR-100 overexpression on cardiac structure and function during physiological aging and pathological pressure-overload-induced heart failure in mice after transverse aortic constriction surgery. MiR-100 was moderately upregulated after induction of pressure overload in mice. While in our transgenic model the cardiomyocyte-specific overexpression of miR-100 did not result in an obvious cardiac phenotype in unchallenged mice, the transgenic mouse strain exhibited less left ventricular dilatation and a higher ejection fraction than wildtype animals, demonstrating an attenuation of maladaptive cardiac remodeling by miR-100. Cardiac transcriptome analysis identified a repression of several regulatory genes related to cardiac metabolism, lipid peroxidation, and production of reactive oxygen species (ROS) by miR-100 overexpression, possibly mediating the observed functional effects. While the modulation of ROS-production seemed to be indirectly affected by miR-100 via Alox5-and Nox4-downregulation, we demonstrated that miR-100 induced a direct repression of the scavenger protein CD36 in murine hearts resulting in a decreased uptake of long-chain fatty acids and an alteration of mitochondrial respiratory function with an enhanced glycolytic state. In summary, we identified miR-100 as a modulator of cardiac metabolism and ROS production without an apparent cardiac phenotype at baseline but a protective effect under conditions of pressure-overload-induced cardiac stress, providing new insight into the mechanisms of heart failure.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- CD36 Antigens - genetics, metabolism
- Disease Models, Animal - administration & dosage
- Fatty Acids - metabolism
- HEK293 Cells - administration & dosage
- Heart Failure - genetics, metabolism
- Humans - administration & dosage
- Male - administration & dosage
- Mice - administration & dosage
- Mice, Inbred C57BL - administration & dosage
- Mice, Transgenic - administration & dosage
- MicroRNAs - genetics, metabolism
- Myocytes, Cardiac - metabolism
- NADPH Oxidase 4 - genetics, metabolism
- Rats - administration & dosage
- Reactive Oxygen Species - metabolism
- Signal Transduction - genetics
- Stroke Volume - genetics
- Transfection - administration & dosage
- Ventricular Remodeling - genetics
- Find related publications in this database (Keywords)
- cardiac metabolism
- CD36
- fatty acid
- miRNA-100
- pressure-overload-induced heart failure