Medizinische Universität Graz - Research portal
Navigation/Search
- Researchers
- Institutions
- Projects
- Publications
- Partners
- Sponsors
- Equipment
- Knowhow
- Fields of Research
"Faces" of the day:
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Vvedenskaya, O; Rose, TD; Knittelfelder, O; Palladini, A; Wodke, JAH; Schuhmann, K; Ackerman, JM; Wang, YT; Has, C; Brosch, M; Thangapandi, VR; Buch, S; Zullig, T; Hartler, J; Kofeler, HC; Rocken, C; Coskun, U; Klipp, E; von Schoenfels, W; Gross, J; Schafmayer, C; Hampe, J; Pauling, JK; Shevchenko, A.
Nonalcoholic fatty liver disease stratification by liver lipidomics
J LIPID RES. 2021; 62: 100104
Doi: 10.1016/j.jlr.2021.100104
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Köfeler Harald
- Züllig Thomas
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Nonalcoholic fatty liver disease (NAFLD) is a common metabolic dysfunction leading to hepatic steatosis. However, NAFLD's global impact on the liver lipidome is poorly understood. Using high-resolution shotgun mass spectrometry, we quanti-fied the molar abundance of 316 species from 22 ma-jor lipid classes in liver biopsies of 365 patients, including nonsteatotic patients with normal or excessive weight, patients diagnosed with NAFL (nonalcoholic fatty liver) or NASH (nonalcoholic steatohepatitis), and patients bearing common muta-tions of NAFLD-related protein factors. We confirmed the progressive accumulation of di-and triacylglycerols and cholesteryl esters in the liver of NAFL and NASH patients, while the bulk composi-tion of glycerophospho-and sphingolipids remained unchanged. Further stratification by biclustering analysis identified sphingomyelin species comprising n24:2 fatty acid moieties as membrane lipid markers of NAFLD. Normalized relative abundance of sphin-gomyelins SM 43:3;2 and SM 43:1;2 containing n24:2 and n24:0 fatty acid moieties, respectively, showed opposite trends during NAFLD progression and distinguished NAFL and NASH lipidomes from the lipidome of nonsteatotic livers. Together with several glycerophospholipids containing a C22:6 fatty acid moiety, these lipids serve as markers of early and advanced stages of NAFL.
- Find related publications in this database (Keywords)
- NAFLD
- shotgun lipidomics
- lipid biomarkers
- sphingomyelins
- liver biopsies
- NAFL
- NASH
- biclustering analysis
- steatosis
- liver lipidome