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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Greimel, TM; Stampfer, L; Leitner, E; Kienesberger, S; Zechner, EL; Bozic, M; Wagner, GE; Unterhauser, K; Kitsera, M; Hauer, AC; Gorkiewicz, G; Wurm, P; Valitutti, F; Högenauer, C; Hoffmann, KM.
Toxin-Producing Klebsiella oxytoca in Healthy Infants: Commensal or Pathobiont?
J Pediatr Gastroenterol Nutr. 2022; 74(1):e1-e7 Doi: 10.1097/MPG.0000000000003299
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Führende Autor*innen der Med Uni Graz
Bauer Theresa Margarete
Hoffmann Karl Martin
Co-Autor*innen der Med Uni Graz
Bozic Michael
Gorkiewicz Gregor
Hauer Almuthe
Hoegenauer Christoph
Kienesberger-Feist Sabine
Leitner-Meyer Eva
Stampfer Laura
Wagner-Lichtenegger Gabriel
Wurm Philipp
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Abstract:
OBJECTIVES: Klebsiella oxytoca is a gastrointestinal pathobiont with the potential to produce the toxins tilivalline and tilimycin, which cause antibiotic-associated hemorrhagic colitis. Overgrowth of toxigenic K oxytoca has recently been implicated in necrotizing enterocolitis. K oxytoca colonizes 2-9% of healthy adults, however, there is no systematic data on colonization in healthy children. We investigated K oxytoca colonization and its toxigenic properties in healthy infants. METHODS: We sampled stool of healthy infants and determined K oxytoca colonization using stool culture and PCR (pehX). Toxin in stool was measured with HPLC/high-resolution mass spectrometry. K oxytoca isolates were typed using multi-locus sequence typing (MLST) and K oxytoca toxin PCR (npsA/B). Cytotoxin production of isolates was analyzed by MTT assay. RESULTS: K oxytoca was detected in 30 of 61 infants (49%) using stool culture and in 45 of 61 (73%) using PCR (pehX). Toxin marker PCR (npsA/B) was positive in 66% of stool samples positive for K oxytoca PCR. Stool toxin levels were too low for quantitation but traces of tilivalline were detected. Contrarily, 49% of K oxytoca isolates demonstrated toxicity in the MTT assay. MLST revealed 36 distinct sequence types affiliated with all known K oxytoca sequence type clusters (A, B1 and B2). CONCLUSIONS: More than 70% of healthy infants were colonized with K oxytoca. Toxin quantities in stool of colonized healthy infants were below detection level, yet half of the isolates produced toxin in vitro demonstrating their pathobiont potential. The high occurrence of toxigenic K oxytoca in healthy infants has to be considered for future disease association studies.

Find related publications in this database (Keywords)
bacteria
cytotoxins
infants
Klebsiella oxytoca
microbiome
pathobiont
pediatrics
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