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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Gauster, M; Rechberger, G; Sovic, A; Hörl, G; Steyrer, E; Sattler, W; Frank, S.
Endothelial lipase releases saturated and unsaturated fatty acids of high density lipoprotein phosphatidylcholine.
J LIPID RES. 2005; 46: 1517-1525.
Doi: 10.1194/jlr.M500054-JLR200
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- Führende Autor*innen der Med Uni Graz
- Frank Sasa
- Gauster Martin
- Co-Autor*innen der Med Uni Graz
- Hörl Gerd
- Sattler Wolfgang
- Steyrer Ernst
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- Abstract:
- We assessed the ability of endothelial lipase (EL) to hydrolyze the sn-1 and sn-2 fatty acids (FAs) from HDL phosphatidylcholine. For this purpose, reconstituted discoidal HDLs (rHDLs) that contained free cholesterol, apolipoprotein A-I, and either 1-palmitoyl-2-oleoylphosphatidylcholine, 1-palmitoyl-2-linoleoylphosphatidylcholine, or 1-palmitoyl-2-arachidonylphosphatidylcholine were incubated with EL- and control (LacZ)-conditioned media. Gas chromatography analysis of the reaction mixtures revealed that both the sn-1 (16:0) and sn-2 (18:1, 18:2, and 20:4) FAs were liberated by EL. The higher rate of sn-1 FA cleavage compared with sn-2 FA release generated corresponding sn-2 acyl lyso-species as determined by MS analysis. EL failed to release sn-2 FA from rHDLs containing 1-O-1'-hexadecenyl-2-arachidonoylphosphatidylcholine, whose sn-1 position contained a nonhydrolyzable alkyl ether linkage. The lack of phospholipase A(2) activity of EL and its ability to liberate [(14)C]FA from [(14)C]lysophosphatidylcholine (lyso-PC) led us to conclude that EL-mediated deacylation of phosphatidylcholine (PC) is initiated at the sn-1 position, followed by the release of the remaining FA from the lyso-PC intermediate. Thin-layer chromatography analysis of cellular lipids obtained from EL-overexpressing cells revealed a pronounced accumulation of [(14)C]phospholipid and [(14)C]triglyceride upon incubation with 1-palmitoyl-2-[1-(14)C]linoleoyl-PC-labeled HDL(3), indicating the ability of EL to supply cells with unsaturated FAs.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- COS Cells -
- Cercopithecus aethiops -
- Fatty Acids, Unsaturated - metabolism
- Humans - metabolism
- Lipase - metabolism
- Lipoproteins, HDL - metabolism
- Lysophospholipase - metabolism
- Phosphatidylcholines - metabolism
- Research Support, Non-U.S. Gov't - metabolism
- Find related publications in this database (Keywords)
- phospholipases
- lysophospholipases
- phospholipids
- polyunsaturated fatty acid
- reconstituted high density lipoprotein