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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Jerkovic, L; Voegele, AF; Chwatal, S; Kronenberg, F; Radcliffe, CM; Wormald, MR; Lobentanz, EM; Ezeh, B; Eller, P; Dejori, N; Dieplinger, B; Lottspeich, F; Sattler, W; Uhr, M; Mechtler, K; Dwek, RA; Rudd, PM; Baier, G; Dieplinger, H.
Afamin is a novel human vitamin E-binding glycoprotein characterization and in vitro expression.
J PROTEOME RES 2005 4: 889-899. Doi: 10.1021/pr0500105
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Co-Autor*innen der Med Uni Graz
Sattler Wolfgang
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Abstract:
Hydrophobic vitamins are transported in human plasma and extravascular fluids by carrier proteins. No specific protein has been described so far for vitamin E, which plays a crucial role in protecting against oxidative damage and disease. We report here the purification of a 75-kDa glycoprotein with vitamin E-binding properties by stepwise chromatography of lipoprotein-depleted human plasma and monitoring of vitamin E (alpha-tocopherol)-binding activity. Partial sequencing identified this protein as afamin, a previously described member of the albumin gene family with four or five potential N-glycosylation sites. Glycosylation analysis indicated that >90% of the glycans were sialylated biantennary complex structures. The vitamin E-binding properties were confirmed using recombinantly expressed afamin. Qualitative and quantitative analysis of plasma and extravascular fluids revealed an abundant presence of this protein not only in plasma (59.8+/-13.3 microg/mL) but also in extravascular fluids such as follicular (34.4+/-12.7 microg/mL) and cerebrospinal (0.28+/-0.16 microg/mL) fluids, suggesting potential roles for afamin in fertility and neuroprotection. Afamin is partly (13%) bound to plasma lipoproteins. Afamin and vitamin E concentrations significantly correlate in follicular and cerebrospinal fluids but not in plasma. The vitamin E association of afamin in follicular fluid was directly demonstrated by gel filtration chromatography and immunoprecipitation which complements the in vitro findings for purified native and recombinant afamin.
Find related publications in this database (using NLM MeSH Indexing)
Biological Transport -
Body Fluids - chemistry
Carrier Proteins - chemistry
Chromatography - chemistry
Glycoproteins - chemistry
Glycosylation - chemistry
Humans - chemistry
Immunoprecipitation - chemistry
Lipoproteins - metabolism
Protein Binding - metabolism
Research Support, Non-U.S. Gov't - metabolism
Serum Albumin - chemistry
Vitamin E - metabolism

Find related publications in this database (Keywords)
afamin
vitamin E binding
glycosylation analysis
2-D electrophoresis
recombinant expression
quantification
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