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Nina Höller

Nariae Baik-Schneditz

Bernhard Schwaberger

Lukas Peter Mileder

Niels Hammer

Gerhard Pichler

Roland Malli

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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Kasatkina, LA; Gumenyuk, VP; Sturm, EM; Heinemann, A; Bernas, T; Trikash, IO.
Modulation of neurosecretion and approaches for its multistep analysis.
Biochim Biophys Acta Gen Subj. 2018; 1862(12):2701-2713 Doi: 10.1016/j.bbagen.2018.08.004
Web of Science PubMed FullText FullText_MUG

Co-Autor*innen der Med Uni Graz: Böhm Eva; Heinemann Akos
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Abstract:: Neurosecretion is the multistep process occurring in separate spatial and temporal cellular boundaries which complicates its comprehensive analysis. Most of the research are focused on one distinct stage of synaptic vesicle recycling. Here, we describe approaches for complex analysis of synaptic vesicle (SV) endocytosis and separate steps of exocytosis at the level of presynaptic bouton and highly purified SVs. Proposed fluorescence-based strategies and analysis of neurotransmitter transport provided the advantages in studies of exocytosis steps. We evaluated SV docking/tethering, their Ca²⁺-dependent fusion and release of neurotransmitters gamma-aminobutyric acid (GABA) and glutamate in two animal models. Approaches enabled us to study: 1) endocytosis/Ca²⁺-dependent release of fluorescent carbon nanodots (CNDs) during stimulation of nerve terminals; 2) the action of levetiracetam, modulator of SV glycoprotein SV2, on fusion competence of SVs and stimulated release of GABA and glutamate; 3) impairments of several steps of neurosecretion under vitamin D₃ deficiency. Our algorithm enabled us to verify the method validity for multidimensional analysis of SV turnover. By increasing SV docking and the size of readily releasable pool (RRP), levetiracetam is able to selectively enhance the stimulated GABA secretion in hippocampal neurons. Findings suggest that SV2 regulates RRP through impact on the number of docked/primed SVs. Methodology can be widely applied to study the stimulated neurosecretion in presynapse, regulation of SV docking, their Ca²⁺-dependent fusion with target membranes, quantitative analysis of expression of neuron-specific proteins, as well as for testing the efficiency of pre-selected designed neuroactive substances. Copyright © 2018 Elsevier B.V. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing): Animals -; Anticonvulsants - pharmacology; Cholecalciferol - deficiency; Endocytosis -; Exocytosis -; Fluorescence -; Hippocampus - drug effects; Hippocampus - metabolism; Levetiracetam - pharmacology; Models, Animal -; Neurosecretion - drug effects; Presynaptic Terminals - drug effects; Presynaptic Terminals - metabolism; Rats -; Synaptic Transmission - physiology; Synaptic Vesicles - metabolism; Vitamin D Deficiency - physiopathology; gamma-Aminobutyric Acid - metabolism
Find related publications in this database (Keywords): Synaptic vesicles recycling; Confocal imaging; Carbon nanodots; Vitamin D-3 deficiency; SV2 protein; GABA release