Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Direkt zur Navigaton springen.
Direkt zum Inhalt springen.

Navigation/Suche

"Köpfe" der aktuellen Meldungen:

Nina Höller

Nariae Baik-Schneditz

Bernhard Schwaberger

Lukas Peter Mileder

Niels Hammer

Gerhard Pichler

Roland Malli

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Magnes, C; Sinner, FM; Regittnig, W; Pieber, TR.
LC/MS/MS method for quantitative determination of long-chain fatty acyl-CoAs.
Anal Chem. 2005; 77(9):2889-2894 Doi: 10.1021/ac048314i
Web of Science PubMed FullText FullText_MUG Google Scholar

Führende Autor*innen der Med Uni Graz: Pieber Thomas
Co-Autor*innen der Med Uni Graz: Regittnig Werner; Sinner Frank Michael
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: Long-chain acyl-CoA esters (LCACoAs) are activated lipid species that represent key substrates in lipid metabolism. The relationship between lipid metabolism disorders and type 2 diabetes has attracted much attention to this class of metabolites. This paper presents a highly sensitive and robust on-line LC/MS(2) procedure for quantitative determination of LCACoAs from rat liver. A fast SPE method has been developed without the need for time-consuming evaporation steps for sample preparation. LCACoAs were separated with high resolution using a C18 reversed-phase column at high pH (10.5) with an ammonium hydroxide and acetonitrile gradient. Five LCACoAs (C16:0, C16:1, C18:0 C18:1, C18:2) were quantified by selective multireaction monitoring using a triple quadrupole mass spectrometer in positive electrospray ionization mode. It is possible to perform a neutral loss scan of 507 for lipid profiling of complex LCACoA mixtures in tissue extracts. The method presented was validated according to ICH guidelines for quantitative determination of five LCACoAs for physiological concentrations in 100-200 mg of tissue with accuracies ranging from 94.8 to 110.8%, interrun precisions between 2.6 and 12.2%, and intrarun precisions between 1.2 and 4.4%. Due to the high sensitivity of the developed method, the amount of tissue biopsied for reliable quantification can be reduced. This may be advantageous in the quantification of LCACoAs in humans.
Find related publications in this database (using NLM MeSH Indexing): Acyl Coenzyme A - analysis; Animals -; Chromatography, High Pressure Liquid -; Liver - chemistry; Male -; Rats -; Rats, Wistar -; Reproducibility of Results -; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods