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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bischof, H; Rehberg, M; Stryeck, S; Artinger, K; Eroglu, E; Waldeck-Weiermair, M; Gottschalk, B; Rost, R; Deak, AT; Niedrist, T; Vujic, N; Lindermuth, H; Prassl, R; Pelzmann, B; Groschner, K; Kratky, D; Eller, K; Rosenkranz, AR; Madl, T; Plesnila, N; Graier, WF; Malli, R.
Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo.
Nat Commun. 2017; 8(1):1422-1422
Doi: 10.1038/s41467-017-01615-z
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- Führende Autor*innen der Med Uni Graz
- Bischof Helmut
- Malli Roland
- Co-Autor*innen der Med Uni Graz
- Artinger Katharina
- Deak Andras Tamas
- Eller Kathrin
- EROGLU Emrah
- Gottschalk Benjamin
- Graier Wolfgang
- Groschner Klaus
- Kratky Dagmar
- Lindermuth Hanna
- Madl Tobias
- Niedrist Tobias Josef
- Pelzmann Brigitte
- Prassl Ruth
- Rosenkranz Alexander
- Rost René
- Stryeck Sarah
- Vujic Nemanja
- Waldeck-Weiermair Markus
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- Abstract:
- Changes in intra- and extracellular potassium ion (K+) concentrations control many important cellular processes and related biological functions. However, our current understanding of the spatiotemporal patterns of physiological and pathological K+ changes is severely limited by the lack of practicable detection methods. We developed K+-sensitive genetically encoded, Förster resonance energy transfer-(FRET) based probes, called GEPIIs, which enable quantitative real-time imaging of K+ dynamics. GEPIIs as purified biosensors are suitable to directly and precisely quantify K+ levels in different body fluids and cell growth media. GEPIIs expressed in cells enable time-lapse and real-time recordings of global and local intracellular K+ signals. Hitherto unknown Ca2+-triggered, organelle-specific K+ changes were detected in pancreatic beta cells. Recombinant GEPIIs also enabled visualization of extracellular K+ fluctuations in vivo with 2-photon microscopy. Therefore, GEPIIs are relevant for diverse K+ assays and open new avenues for live-cell K+ imaging.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Biosensing Techniques - methods
- Computer Systems -
- Extracellular Fluid - metabolism
- Fluorescence Resonance Energy Transfer -
- Fluorescent Dyes -
- Green Fluorescent Proteins - genetics
- Green Fluorescent Proteins - metabolism
- HeLa Cells -
- Humans -
- In Vitro Techniques -
- Intracellular Fluid - metabolism
- Male -
- Mice -
- Mice, Inbred C57BL -
- Microscopy, Fluorescence, Multiphoton -
- Potassium - blood
- Potassium - metabolism
- Potassium - urine