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Kawala, MA; Bohndorf, M; Graffmann, N; Wruck, W; Zatloukal, K; Adjaye, J.
Characterization of iPSCs derived from dermal fibroblasts from a healthy 19year old female.
Stem Cell Res. 2016; 17(3):597-599
Doi: 10.1016/j.scr.2016.10.002
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- Co-authors Med Uni Graz
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Zatloukal Kurt
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Primary fibroblasts from a healthy 19years old female were reprogrammed by transduction of retroviruses OCT4, SOX2, c-MYC and KLF4. iPSCs were characterized by immunocytochemistry, embryonic body-formation, DNA-fingerprint and karyotype analysis and comparative transcriptome analyses with the human embryonic stem cell line H1 revealed a Pearsons correlation coefficient of 0.8952.
Copyright © 2016 Michael Boutros, German Cancer Research Center, Heidelberg, Germany. Published by Elsevier B.V. All rights reserved.
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Cell Differentiation -
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Cell Line -
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Cellular Reprogramming -
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Comparative Genomic Hybridization -
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DNA Fingerprinting -
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Dermis - cytology
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Embryoid Bodies - cytology
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Embryoid Bodies - metabolism
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Female -
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Fibroblasts - cytology
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Genetic Vectors - genetics
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Genetic Vectors - metabolism
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Healthy Volunteers -
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Human Embryonic Stem Cells - cytology
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Human Embryonic Stem Cells - metabolism
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Humans -
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Induced Pluripotent Stem Cells - cytology
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Induced Pluripotent Stem Cells - metabolism
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Karyotype -
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Microscopy, Fluorescence -
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Retroviridae - genetics
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Transcription Factors - genetics
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Transcription Factors - metabolism
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Young Adult -