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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Amplatz, B; Zöhrer, E; Haas, C; Schäffer, M; Stojakovic, T; Jahnel, J; Fauler, G.
Bile acid preparation and comprehensive analysis by high performance liquid chromatography-high-resolution mass spectrometry.
Clin Chim Acta. 2017; 464(2):85-92
Doi: 10.1016/j.cca.2016.11.014
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Fauler Günter
- Zöhrer Evelyn
- Co-Autor*innen der Med Uni Graz
- Jahnel Jörg
- Stojakovic Tatjana
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- Abstract:
- Accurate analytical methods for bile acid (BA) analysis are important for clinical diagnosis in newborns, adolescents, and adults. Improvements in speed, sensitivity and simplicity enable BA profiling using high performance liquid chromatography (HPLC) together with electrospray ionization (ESI) and high-resolution mass spectrometry (HR-MS). We present a method, validated in different species and tissues, enabling a highly sensitive quantitative determination (in a range of 0.24pmol/sample to 1000pmol/sample, corresponding to 0.024-100pmol on column) of up to 36 naturally occurring BAs from as little as 10μl of plasma or serum, 1ml of urine, or 10mg of dried stool. Chromatographic separation is achieved by HPLC using a C18 reversed phase column and a water/methanol gradient. After ESI, BAs are analyzed through HR-MS using orbitrap technology in full scan mode. 30 different BAs and the corresponding internal standards are separated and analyzed in a single run. Six additional BAs are evaluated in a second run using a pentafluorophenyl (PFP) stationary phase. This method generates detailed human and rodent BA profiles in full scan mode and accurate mass with the advantage of remarkably low required sample volume. Copyright © 2016 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Analytic Sample Preparation Methods - methods
- Bile Acids and Salts - analysis
- Bile Acids and Salts - blood
- Bile Acids and Salts - isolation & purification
- Bile Acids and Salts - urine
- Child -
- Chromatography, High Pressure Liquid - methods
- Clinical Chemistry Tests - methods
- Humans -
- Infant, Newborn -
- Reference Standards -
- Reproducibility of Results -
- Spectrometry, Mass, Electrospray Ionization - methods
- Find related publications in this database (Keywords)
- Bile acids
- Muricholic acids
- High performance liquid chromatography
- High resolution-mass spectrometry
- Neonates