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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Eroglu, E; Gottschalk, B; Charoensin, S; Blass, S; Bischof, H; Rost, R; Madreiter-Sokolowski, CT; Pelzmann, B; Bernhart, E; Sattler, W; Hallström, S; Malinski, T; Waldeck-Weiermair, M; Graier, WF; Malli, R.
Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics.
Nat Commun. 2016; 7(3):10623-10623
Doi: 10.1038/ncomms10623
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- Führende Autor*innen der Med Uni Graz
- EROGLU Emrah
- Malli Roland
- Co-Autor*innen der Med Uni Graz
- Bernhart Eva Maria
- Bischof Helmut
- Blass Sandra
- Gottschalk Benjamin
- Graier Wolfgang
- Hallström Seth
- Madreiter-Sokolowski Corina
- Pelzmann Brigitte
- Rost René
- Sattler Wolfgang
- Waldeck-Weiermair Markus
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- Abstract:
- Nitric oxide () is a free radical with a wide range of biological effects, but practically impossible to visualize in single cells. Here we report the development of novel multicoloured fluorescent quenching-based probes by fusing a bacteria-derived -binding domain close to distinct fluorescent protein variants. These genetically encoded probes, referred to as geNOps, provide a selective, specific and real-time read-out of cellular dynamics and, hence, open a new era of bioimaging. The combination of geNOps with a Ca(2+) sensor allowed us to visualize and Ca(2+) signals simultaneously in single endothelial cells. Moreover, targeting of the probes was used to detect signals within mitochondria. The geNOps are useful new tools to further investigate and understand the complex patterns of signalling on the single (sub)cellular level.
- Find related publications in this database (using NLM MeSH Indexing)
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