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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Roier, S; Zingl, FG; Cakar, F; Durakovic, S; Kohl, P; Eichmann, TO; Klug, L; Gadermaier, B; Weinzerl, K; Prassl, R; Lass, A; Daum, G; Reidl, J; Feldman, MF; Schild, S.
A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria.
Nat Commun. 2016; 7:10515
Doi: 10.1038/ncomms10515
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- Co-Autor*innen der Med Uni Graz
- Eichmann Thomas
- Prassl Ruth
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- Abstract:
- Bacterial outer membrane vesicles (OMVs) have important biological roles in pathogenesis and intercellular interactions, but a general mechanism of OMV formation is lacking. Here we show that the VacJ/Yrb ABC (ATP-binding cassette) transport system, a proposed phospholipid transporter, is involved in OMV formation. Deletion or repression of VacJ/Yrb increases OMV production in two distantly related Gram-negative bacteria, Haemophilus influenzae and Vibrio cholerae. Lipidome analyses demonstrate that OMVs from VacJ/Yrb-defective mutants in H. influenzae are enriched in phospholipids and certain fatty acids. Furthermore, we demonstrate that OMV production and regulation of the VacJ/Yrb ABC transport system respond to iron starvation. Our results suggest a new general mechanism of OMV biogenesis based on phospholipid accumulation in the outer leaflet of the outer membrane. This mechanism is highly conserved among Gram-negative bacteria, provides a means for regulation, can account for OMV formation under all growth conditions, and might have important pathophysiological roles in vivo.
- Find related publications in this database (using NLM MeSH Indexing)
- ATP-Binding Cassette Transporters - physiology
- Animals - administration & dosage
- Cytoplasmic Vesicles - physiology
- Escherichia coli - administration & dosage
- Female - administration & dosage
- Haemophilus influenzae - physiology
- Mice, Inbred BALB C - administration & dosage
- Organelle Biogenesis - administration & dosage
- Vibrio cholerae - physiology