Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Navigation/Suche
- Forscher*innen
- Institutionen
- Projekte
- Publikationen
- Partner
- Geldgeber
- Ausstattung
- Knowhow
- Forschungsfelder
"Köpfe" der aktuellen Meldungen:
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Sala, P; Pötz, S; Brunner, M; Trötzmüller, M; Fauland, A; Triebl, A; Hartler, J; Lankmayr, E; Köfeler, HC.
Determination of oxidized phosphatidylcholines by hydrophilic interaction liquid chromatography coupled to Fourier transform mass spectrometry.
Int J Mol Sci. 2015; 16(4):8351-8363
Doi: 10.3390/ijms16048351
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Köfeler Harald
- Co-Autor*innen der Med Uni Graz
- Brunner Martina
- Hartler Jürgen
- Triebl Alexander
- Trötzmüller Martin
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- A novel liquid chromatography-mass spectrometry (LC-MS) approach for analysis of oxidized phosphatidylcholines by an Orbitrap Fourier Transform mass spectrometer in positive electrospray ionization (ESI) coupled to hydrophilic interaction liquid chromatography (HILIC) was developed. This method depends on three selectivity criteria for separation and identification: retention time, exact mass at a resolution of 100,000 and collision induced dissociation (CID) fragment spectra in a linear ion trap. The process of chromatography development showed the best separation properties with a silica-based Kinetex column. This type of chromatography was able to separate all major lipid classes expected in mammalian samples, yielding increased sensitivity of oxidized phosphatidylcholines over reversed phase chromatography. Identification of molecular species was achieved by exact mass on intact molecular ions and CID tandem mass spectra containing characteristic fragments. Due to a lack of commercially available standards, method development was performed with copper induced oxidation products of palmitoyl-arachidonoyl-phosphatidylcholine, which resulted in a plethora of lipid species oxidized at the arachidonoyl moiety. Validation of the method was done with copper oxidized human low-density lipoprotein (LDL) prepared by ultracentrifugation. In these LDL samples we could identify 46 oxidized molecular phosphatidylcholine species out of 99 possible candidates.
- Find related publications in this database (using NLM MeSH Indexing)
- Chromatography, High Pressure Liquid -
- Fourier Analysis -
- Humans -
- Hydrophobic and Hydrophilic Interactions -
- Lipoproteins, LDL - chemistry
- Oxidation-Reduction -
- Phosphatidylcholines - chemistry
- Spectrometry, Mass, Electrospray Ionization -
- Tandem Mass Spectrometry -
- Find related publications in this database (Keywords)
- fourier transform orbitrap mass spectrometry
- hydrophilic interaction liquid chromatography
- oxidized low density lipoprotein
- oxidized phospholipids
- LC-MS