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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Karbiener, M; Fischer, C; Nowitsch, S; Opriessnig, P; Papak, C; Ailhaud, G; Dani, C; Amri, EZ; Scheideler, M.
microRNA miR-27b impairs human adipocyte differentiation and targets PPARgamma.
BIOCHEM BIOPH RES CO. 2009; 390(2): 247-251.
Doi: 10.1016/j.bbrc.2009.09.098
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Karbiener Michael
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- Abstract:
- Obesity has emerged as a global health problem with more than 1.1 billion adults to be classified as overweight or obese, and is associated with type 2 diabetes, cardiovascular disease, and several cancers. Since obesity is characterized by an increased size and/or number of adipocytes, elucidating the molecular events governing adipogenesis is of utmost importance. Recent findings indicate that microRNAs (miRNAs) - small non-protein-coding RNAs that function as post-transcriptional gene regulators - are involved in the regulatory network of adipogenesis. Whereas only a single human miRNA is known so far to be functional in adipogenesis as pro-adipogenic, several mouse miRNAs have been identified very recently as adipogenic regulators, thereby stimulating demand for studying the functional role of miRNAs during adipogenesis in human. Here, we demonstrate that miR-27b abundance decreased during adipogenesis of human multipotent adipose-derived stem (hMADS) cells. Overexpression of miR-27b blunted induction of PPARgamma and C/EBPalpha, two key regulators of adipogenesis, during early onset of adipogenesis and repressed adipogenic marker gene expression and triglyceride accumulation at late stages. PPARgamma has a predicted and highly conserved binding site in its 3'UTR and was indeed confirmed to be a direct target of miR-27b. Thus, these results suggest that the anti-adipogenic effect of miR-27b in hMADS cells is due, at least in part, to suppression of PPARgamma.
- Find related publications in this database (using NLM MeSH Indexing)
- 3' Untranslated Regions - metabolism
- Adipocytes - metabolism Adipocytes - physiology
- Adipogenesis - genetics
- Animals -
- Base Sequence -
- CCAAT-Enhancer-Binding Protein-alpha - metabolism
- Gene Expression Regulation -
- Humans -
- Mice -
- MicroRNAs - metabolism
- PPAR gamma - genetics PPAR gamma - metabolism
- Find related publications in this database (Keywords)
- microRNA
- miR-27b
- Adipogenesis
- PPAR gamma