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"Faces" of the day:

Katharina Jandl

Nina Höller

Nariae Baik-Schneditz

Ellen Heitzer

Marianne Brodmann

Ewald Kolesnik

Bernhard Schwaberger

Maximilian Seles

Gabor Toth-Gayor

Lukas Peter Mileder

Niels Hammer

Christian Josef Hill

Christian Viertler

Philipp Klaritsch

Daniel Scherr

Harald Köfeler

Gerhard Pichler

Roland Malli

Michael Fuchsjäger

Gernot Plank

Peter Fickert

Richard Zigeuner

Peter Holzer

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Konya, V; Peinhaupt, M; Heinemann, A.
Adhesion of eosinophils to endothelial cells or substrates under flow conditions.
Methods Mol Biol. 2014; 1178(5):143-156 Doi: 10.1007/978-1-4939-1016-8_13
PubMed FullText FullText_MUG

 

Leading authors Med Uni Graz

Heinemann Akos

Konya Viktoria

Co-authors Med Uni Graz

Peinhaupt Miriam

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Abstract:

Recruitment of eosinophils into the lung tissue is a critical event in allergic inflammatory reactions. Extravasation of eosinophils from the bloodstream is a highly dynamic multistep process that involves capture, rolling, activation, firm adhesion, and transendothelial and subendothelial migration of the cells. It is assumed that the rate-limiting step in this cascade is the capture and firm adhesion of cells to the endothelium. As such it is most critical to study endothelial-leukocyte interaction using assays which are capable of mimicking physiological flow conditions. Previously, various parallel flow chamber setups had been used for studying leukocyte adhesion to endothelial cells. Here we describe a highly reproducible technique for investigating eosinophil adhesion to endothelial cell layer or adhesion molecule/extracellular matrix protein coating in biochips by using a semiautomated microfluidic platform and live-cell imaging. In detail, we show eosinophil adhesion to endothelial cells activated by tumour necrosis factor (TNF) alpha, and adhesion to fibronectin of eosinophils stimulated by prostaglandin (PG) D2.

Find related publications in this database (using NLM MeSH Indexing)

Cell Adhesion - physiology

Endothelial Cells - cytology

Endothelial Cells - metabolism

Endothelial Cells - physiology

Eosinophils - cytology

Eosinophils - metabolism

Eosinophils - physiology

Fibronectins - metabolism

Humans -

Tumor Necrosis Factor-alpha - metabolism

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