Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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"Köpfe" der aktuellen Meldungen:

Nina Höller

Nariae Baik-Schneditz

Bernhard Schwaberger

Lukas Peter Mileder

Niels Hammer

Daniel Scherr

Gerhard Pichler

Roland Malli

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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Konya, V; Peinhaupt, M; Heinemann, A.
Adhesion of eosinophils to endothelial cells or substrates under flow conditions.
Methods Mol Biol. 2014; 1178(5):143-156 Doi: 10.1007/978-1-4939-1016-8_13
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Führende Autor*innen der Med Uni Graz: Heinemann Akos; Konya Viktoria
Co-Autor*innen der Med Uni Graz: Peinhaupt Miriam
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Abstract:: Recruitment of eosinophils into the lung tissue is a critical event in allergic inflammatory reactions. Extravasation of eosinophils from the bloodstream is a highly dynamic multistep process that involves capture, rolling, activation, firm adhesion, and transendothelial and subendothelial migration of the cells. It is assumed that the rate-limiting step in this cascade is the capture and firm adhesion of cells to the endothelium. As such it is most critical to study endothelial-leukocyte interaction using assays which are capable of mimicking physiological flow conditions. Previously, various parallel flow chamber setups had been used for studying leukocyte adhesion to endothelial cells. Here we describe a highly reproducible technique for investigating eosinophil adhesion to endothelial cell layer or adhesion molecule/extracellular matrix protein coating in biochips by using a semiautomated microfluidic platform and live-cell imaging. In detail, we show eosinophil adhesion to endothelial cells activated by tumour necrosis factor (TNF) alpha, and adhesion to fibronectin of eosinophils stimulated by prostaglandin (PG) D2.
Find related publications in this database (using NLM MeSH Indexing): Cell Adhesion - physiology; Endothelial Cells - cytology; Endothelial Cells - metabolism; Endothelial Cells - physiology; Eosinophils - cytology; Eosinophils - metabolism; Eosinophils - physiology; Fibronectins - metabolism; Humans -; Tumor Necrosis Factor-alpha - metabolism