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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Leis, HJ; Fauler, G; Windischhofer, W.
Enantioselective quantitative analysis of amphetamine in human plasma by liquid chromatography/high-resolution mass spectrometry.
Anal Bioanal Chem. 2014; 406(18):4473-4480
Doi: 10.1007/s00216-014-7850-4
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Leis Hans-Joerg
- Co-Autor*innen der Med Uni Graz
- Fauler Günter
- Windischhofer Werner
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- Abstract:
- A method for the quantitative enantioselective analysis of amphetamine in human plasma by LC-HRMS is presented. High-resolution detection, alone and in combination with targeted MS/MS, was validated and compared to a highly sensitive GC-NICI-Method. Derivatization with (S)-N-(heptafluorobutyryl)-prolyl chloride was accomplished to yield derivatives suitable for enantioselective analysis of amphetamine on a nonchiral reversed phase column with MS-compatible mobile phase. Equal analytical performance was observed for the methods presented and the GC-NICI-MS method. A dynamic range of 4,000 was found for the established calibration curves. A fivefold deuterated analogue of both enantiomeres was used as an internal standard. Full validation data are given to demonstrate the usefulness of the assay, including specificity, linearity, accuracy and precision, autosampler stability, matrix effect, and prospective analytical batch size accuracy. The method has been successfully applied to pharmacokinetic profiling of the drug after oral application.
- Find related publications in this database (using NLM MeSH Indexing)
- Administration, Oral -
- Amphetamine - administration & dosage
- Amphetamine - blood
- Amphetamine - chemistry
- Amphetamine - pharmacokinetics
- Calibration -
- Chromatography, Liquid - methods
- Fluorocarbons - chemistry
- Humans -
- Proline - analogs & derivatives
- Proline - chemistry
- Reproducibility of Results -
- Sensitivity and Specificity -
- Stereoisomerism -
- Tandem Mass Spectrometry - methods
- Find related publications in this database (Keywords)
- Bioanalytical methods
- Chiral analysis
- Drug monitoring
- Drug screening
- HPLC
- Mass spectrometry