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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Klug, L; Tarazona, P; Gruber, C; Grillitsch, K; Gasser, B; Trötzmüller, M; Köfeler, H; Leitner, E; Feussner, I; Mattanovich, D; Altmann, F; Daum, G.
The lipidome and proteome of microsomes from the methylotrophic yeast Pichia pastoris.
Biochim Biophys Acta. 2014; 1841(2):215-226
Doi: 10.1016/j.bbalip.2013.11.005
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Köfeler Harald
- Trötzmüller Martin
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- Abstract:
- The methylotrophic yeast Pichia pastoris is a popular yeast expression system for the production of heterologous proteins in biotechnology. Interestingly, cell organelles which play an important role in this process have so far been insufficiently investigated. For this reason, we started a systematic approach to isolate and characterize organelles from P. pastoris. In this study, we present a procedure to isolate microsomal membranes at high purity. These samples represent endoplasmic reticulum (ER) fractions which were subjected to molecular analysis of lipids and proteins. Organelle lipidomics included a detailed analysis of glycerophospholipids, fatty acids, sterols and sphingolipids. The microsomal proteome analyzed by mass spectrometry identified typical proteins of the ER known from other cell types, especially Saccharomyces cerevisiae, but also a number of unassigned gene products. The lipidome and proteome analysis of P. pastoris microsomes are prerequisite for a better understanding of functions of this organelle and for modifying this compartment for biotechnological applications.
- Find related publications in this database (using NLM MeSH Indexing)
- Fungal Proteins - analysis
- Glycerophospholipids - analysis
- Lipids - analysis
- Microsomes - chemistry
- Pichia - chemistry
- Pichia - ultrastructure
- Proteome - analysis
- Quality Control -
- Sphingolipids - analysis
- Find related publications in this database (Keywords)
- Pichia pastoris
- Endoplasmic reticulum
- Proteome
- Sphingolipids
- Phospholipid
- Fatty acid