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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Pratter, SM; Ivkovic, J; Birner-Gruenberger, R; Breinbauer, R; Zangger, K; Straganz, GD.
More than just a halogenase: modification of fatty acyl moieties by a trifunctional metal enzyme.
Chembiochem. 2014; 15(4):567-574
Doi: 10.1002/cbic.201300345
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
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- Abstract:
- The highly selective oxidative halogenations by non-heme iron and α-ketoglutarate-dependent enzymes are key reactions in the biosynthesis of lipopeptides, and often bestow valuable bioactivity to the metabolites. Here we present the first biochemical characterization of a putative fatty acyl halogenase, HctB, which is found in the hectochlorin biosynthetic pathway of Lyngbya majuscula. Its unprecedented three-domain structure, which includes an acyl carrier protein domain, allows self-contained conversion of the covalently tethered hexanoyl substrate. Structural analysis of the native product by (13) C NMR reveals high regioselectivity but considerable catalytic promiscuity. This challenges the classification of HctB as a primary halogenase: along with the proposed dichlorination, HctB performs oxygenation and an unprecedented introduction of a vinyl-chloride moiety into the nonactivated carbon chain. The relaxed substrate specificity is discussed with reference to a molecular model of the enzyme-substrate complex. The results suggest that fatty acyl transformation at the metal center of HctB can bring about considerable structural diversity in the biosynthesis of lipopeptides. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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- Lactones - chemistry
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- Find related publications in this database (Keywords)
- halogenation
- metalloenzymes
- molecular diversity
- natural products
- non-heme iron