Medizinische Universität Graz - Research portal
Navigation/Search
- Researchers
- Institutions
- Projects
- Publications
- Partners
- Sponsors
- Equipment
- Knowhow
- Fields of Research
"Faces" of the day:
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Karbiener, M; Pisani, DF; Frontini, A; Oberreiter, LM; Lang, E; Vegiopoulos, A; Mössenböck, K; Bernhardt, GA; Mayr, T; Hildner, F; Grillari, J; Ailhaud, G; Herzig, S; Cinti, S; Amri, EZ; Scheideler, M.
MicroRNA-26 family is required for human adipogenesis and drives characteristics of brown adipocytes.
Stem Cells. 2014; 32(6):1578-1590
Doi: 10.1002/stem.1603
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Karbiener Michael
- Co-authors Med Uni Graz
- Bernhardt Gerwin
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Adipose tissue contains thermogenic adipocytes (i.e., brown and brite/beige) that oxidize nutrients at exceptionally high rates via nonshivering thermogenesis. Its recent discovery in adult humans has opened up new avenues to fight obesity and related disorders such as diabetes. Here, we identified miR-26a and -26b as key regulators of human white and brite adipocyte differentiation. Both microRNAs are upregulated in early adipogenesis, and their inhibition prevented lipid accumulation while their overexpression accelerated it. Intriguingly, miR-26a significantly induced pathways related to energy dissipation, shifted mitochondrial morphology toward that seen in brown adipocytes, and promoted uncoupled respiration by markedly increasing the hallmark protein of brown fat, uncoupling protein 1. By combining in silico target prediction, transcriptomics, and an RNA interference screen, we identified the sheddase ADAM metallopeptidase domain 17 (ADAM17) as a direct target of miR-26 that mediated the observed effects on white and brite adipogenesis. These results point to a novel, critical role for the miR-26 family and its downstream effector ADAM17 in human adipocyte differentiation by promoting characteristics of energy-dissipating thermogenic adipocytes. © 2013 AlphaMed Press.
- Find related publications in this database (using NLM MeSH Indexing)
- ADAM Proteins - metabolism
- Adipocytes, Brown - cytology Adipocytes, Brown - metabolism Adipocytes, Brown - ultrastructure
- Adipogenesis - genetics
- Adipose Tissue, White - metabolism Adipose Tissue, White - ultrastructure
- Adult -
- Cell Differentiation - genetics
- Child, Preschool -
- Cold Temperature -
- Computer Simulation -
- Humans -
- Infant -
- Ion Channels -
- Male -
- MicroRNAs - genetics MicroRNAs - metabolism
- Mitochondria - metabolism Mitochondria - ultrastructure
- Mitochondrial Proteins -
- Signal Transduction - genetics
- Transcriptome - genetics
- Up-Regulation - genetics
- Find related publications in this database (Keywords)
- Adipocyte differentiation
- White adipogenesis
- Brite adipogenesis
- MicroRNA-26
- ADAM17