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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Chitraju, C; Trötzmüller, M; Hartler, J; Wolinski, H; Thallinger, GG; Haemmerle, G; Zechner, R; Zimmermann, R; Köfeler, HC; Spener, F.
The impact of genetic stress by ATGL deficiency on the lipidome of lipid droplets from murine hepatocytes.
J Lipid Res. 2013; 54(8):2185-2194
Doi: 10.1194/jlr.M037952
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- Leading authors Med Uni Graz
- Trötzmüller Martin
- Co-authors Med Uni Graz
- Hartler Jürgen
- Köfeler Harald
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- Abstract:
- We showed earlier that nutritional stress like starvation or high-fat diet resulted in phenotypic changes in the lipidomes of hepatocyte lipid droplets (LDs), representative for the pathophysiological status of the mouse model. Here we extend our former study by adding genetic stress due to knockout (KO) of adipocyte triglyceride lipase (ATGL), the rate limiting enzyme in LD lipolysis. An intervention trial for 6 weeks with male wild-type (WT) and ATGL-KO mice was carried out; both genotypes were fed lab chow or were exposed to short-time starvation. Isolated LDs were analyzed by LC-MS/MS. Triacylglycerol, diacylglycerol, and phosphatidylcholine lipidomes, in that order, provided the best phenotypic signatures characteristic for respective stresses applied to the animals. This was evidenced at lipid species level by principal component analysis, calculation of average values for chain-lengths and numbers of double bonds, and by visualization in heat maps. Structural backgrounds for analyses and metabolic relationships were elaborated at lipid molecular species level. Relating our lipidomic data to nonalcoholic fatty liver diseases of nutritional and genetic etiologies with or without accompanying insulin resistance, phenotypic distinction in hepatocyte LDs dependent on insulin status emerged. Taken together, lipidomes of hepatocyte LDs are sensitive responders to nutritional and genetic stress.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- DNA Damage -
- Gene Deletion -
- Hepatocytes - chemistry
- Lipase - deficiency
- Lipid Metabolism -
- Lipids -
- Male -
- Mice -
- Mice, Inbred C57BL -
- Mice, Knockout -
- Particle Size -
- Find related publications in this database (Keywords)
- adipocyte triglyceride lipase
- fasting
- nutritional stress
- super stress
- steatosis
- mass spectrometry
- Lipid Data Analyzer
- principal component analysis
- lipid species