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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Rao, SP; Riederer, M; Lechleitner, M; Hermansson, M; Desoye, G; Hallström, S; Graier, WF; Frank, S.
Acyl chain-dependent effect of lysophosphatidylcholine on endothelium-dependent vasorelaxation.
PLoS One. 2013; 8(5):e65155-e65155
Doi: 10.1371/journal.pone.0065155
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- Führende Autor*innen der Med Uni Graz
- Frank Sasa
- Rao Shailaja Prabhakar
- Co-Autor*innen der Med Uni Graz
- Desoye Gernot
- Graier Wolfgang
- Hallström Seth
- Lechleitner Margarete
- Riederer Monika
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- Abstract:
- Previously we identified palmitoyl-, oleoyl-, linoleoyl-, and arachidonoyl-lysophosphatidylcholine (LPC 16:0, 18:1, 18:2 and 20:4) as the most prominent LPC species generated by endothelial lipase (EL). In the present study, we examined the impact of those LPC on acetylcholine (ACh)- induced vascular relaxation. All tested LPC attenuated ACh-induced relaxation, measured ex vivo, using mouse aortic rings and wire myography. The rank order of potency was as follows: 18:2>20:4>16:0>18:1. The attenuating effect of LPC 16:0 on relaxation was augmented by indomethacin-mediated cyclooxygenase (COX)-inhibition and CAY10441, a prostacyclin (PGI2)- receptor (IP) antagonist. Relaxation attenuated by LPC 20:4 and 18:2 was improved by indomethacin and SQ29548, a thromboxane A2 (TXA2)- receptor antagonist. The effect of LPC 20:4 could also be improved by TXA2- and PGI2-synthase inhibitors. As determined by EIA assays, the tested LPC promoted secretion of PGI2, TXA2, PGF2α, and PGE2, however, with markedly different potencies. LPC 16:0 was the most potent inducer of superoxide anion production by mouse aortic rings, followed by LPC 18:2, 20:4 and 18:1, respectively. The strong antioxidant tempol recovered relaxation impairment caused by LPC 18:2, 18:1 and 20:4, but not by LPC 16:0. The tested LPC attenuate ACh-induced relaxation through induction of proconstricting prostanoids and superoxide anions. The potency of attenuating relaxation and the relative contribution of underlying mechanisms are strongly related to LPC acyl-chain length and degree of saturation.
- Find related publications in this database (using NLM MeSH Indexing)
- Acetylcholine - pharmacology
- Animals -
- Aorta, Thoracic - drug effects
- Dose-Response Relationship, Drug -
- Endothelium, Vascular - drug effects
- Epoprostenol - metabolism
- In Vitro Techniques -
- Lysophosphatidylcholines - pharmacology
- Male -
- Mice -
- Oxidative Stress - drug effects
- Prostaglandin-Endoperoxide Synthases - metabolism
- Prostaglandins - metabolism
- Receptors, Thromboxane - antagonists & inhibitors
- Thromboxane A2 - metabolism
- Vasodilation - drug effects