Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Navigation/Suche
- Forscher*innen
- Institutionen
- Projekte
- Publikationen
- Partner
- Geldgeber
- Ausstattung
- Knowhow
- Forschungsfelder
"Köpfe" der aktuellen Meldungen:
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Carmona-Gutierrez, D; Alavian-Ghavanini, A; Habernig, L; Bauer, MA; Hammer, A; Rossmann, C; Zimmermann, AS; Ruckenstuhl, C; Büttner, S; Eisenberg, T; Sattler, W; Malle, E; Madeo, F.
The cell death protease Kex1p is essential for hypochlorite-induced apoptosis in yeast.
Cell Cycle. 2013; 12(11):1704-1712
Doi: 10.4161/cc.24801
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Alavian Ghavanini Ali
- Malle Ernst
- Co-Autor*innen der Med Uni Graz
- Hammer Astrid
- Rossmann Christine Renate
- Sattler Wolfgang
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Following microbial pathogen invasion, the human immune system of activated phagocytes generates and releases the potent oxidant hypochlorous acid (HOCl), which contributes to the killing of menacing microorganisms. Though tightly controlled, HOCl generation by the myeloperoxidase-hydrogen peroxide-chloride system of neutrophils/monocytes may occur in excess and lead to tissue damage. It is thus of marked importance to delineate the molecular pathways underlying HOCl cytotoxicity in both microbial and human cells. Here, we show that HOCl induces the generation of reactive oxygen species (ROS), apoptotic cell death and the formation of specific HOCl-modified epitopes in the budding yeast Saccharomyces cerevisiae. Interestingly, HOCl cytotoxicity can be prevented by treatment with ROS scavengers, suggesting oxidative stress to mediate the lethal effect. The executing pathway involves the pro-apoptotic protease Kex1p, since its absence diminishes HOCl-induced production of ROS, apoptosis and protein modification. By characterizing HOCl-induced cell death in yeast and identifying a corresponding central executor, these results pave the way for the use of Saccharomyces cerevisiae in HOCl research, not least given that it combines both being a microorganism as well as a model for programmed cell death in higher eukaryotes.
- Find related publications in this database (using NLM MeSH Indexing)
- Apoptosis - drug effects
- Carboxypeptidases - metabolism
- Epitopes - chemistry
- Hypochlorous Acid - toxicity
- Phosphorylation -
- Reactive Oxygen Species - metabolism
- Saccharomyces cerevisiae - metabolism
- Find related publications in this database (Keywords)
- hypochlorous acid
- HOCl
- apoptosis
- reactive oxygen species
- yeast
- Saccharomyces cerevisiae
- mitochondria