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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Cipak, A; Hasslacher, M; Tehlivets, O; Collinson, EJ; Zivkovic, M; Matijevic, T; Wonisch, W; Waeg, G; Dawes, IW; Zarkovic, N; Kohlwein, SD.
Saccharomyces cerevisiae strain expressing a plant fatty acid desaturase produces polyunsaturated fatty acids and is susceptible to oxidative stress induced by lipid peroxidation.
Free Radic Biol Med. 2006; 40(5): 897-906.
Doi: 10.1016/j.freeradbiomed.2005.10.039
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Tehlivets Oksana
- Wonisch Willibald
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- Abstract:
- Although oxygen is essential for aerobic organisms, it also forms potentially harmful reactive oxygen species. For its simplicity, easy manipulation, and cultivation conditions, yeast is used as an attractive model in oxidative stress research. However, lack of polyunsaturated fatty acids in yeast membranes makes yeast unsuitable for research in the field of lipid peroxidation. Therefore, we have constructed a yeast strain expressing a Delta12 desaturase gene from the tropical rubber tree, Hevea brasiliensis. This yeast strain expresses the heterologous desaturase in an active form and, consequently, produces Delta9/Delta12 polyunsaturated fatty acids under inducing conditions. The functional expression of the heterologous desaturase did not affect cellular morphology or growth, indicating no general adverse effect on cellular physiology. However, the presence of polyunsaturated fatty acids changed the yeast's sensitivity to oxidative stress induced by addition of paraquat, tert-butylhydroperoxide, and hydrogen peroxide. This difference in sensitivity to the latter was followed by the formation of 4-hydroxy-2-nonenal, one of the end products of linoleic fatty acid peroxidation, which is known to play a role in cell growth control and signaling. Here we show that this yeast strain conditionally expressing the Delta12 desaturase gene provides a novel and well-defined eukaryotic model in lipid peroxidation research. Its potential to investigate the molecular basis of responses to oxidative stress, in particular the involvement of reactive aldehydes derived from fatty acid peroxidation, especially 4-hydroxy-2-nonenal, will be addressed.
- Find related publications in this database (using NLM MeSH Indexing)
- Aldehydes - metabolism
- Amino Acid Sequence -
- Cloning, Molecular -
- Fatty Acid Desaturases - genetics Fatty Acid Desaturases - metabolism
- Fatty Acids, Unsaturated - biosynthesis
- Free Radicals - toxicity
- Hevea - enzymology Hevea - genetics
- Hydrogen Peroxide - toxicity
- Lipid Peroxidation -
- Molecular Sequence Data -
- Oxidative Stress -
- Paraquat - toxicity
- Plant Proteins - genetics Plant Proteins - metabolism
- Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - metabolism
- tert-Butylhydroperoxide - toxicity
- Find related publications in this database (Keywords)
- yeast
- lipid oxidation
- PUFA
- oxidative stress
- reactive oxygen species
- 4-hydroxy-2-nonenal
- free radicals