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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ivashov, VA; Grillitsch, K; Koefeler, H; Leitner, E; Baeumlisberger, D; Karas, M; Daum, G.
Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris.
Biochim Biophys Acta. 2013; 1831(2):282-290
Doi: 10.1016/j.bbalip.2012.09.017
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- Co-Autor*innen der Med Uni Graz
- Köfeler Harald
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- Abstract:
- Lipid droplets (LD) are the main depot of non-polar lipids in all eukaryotic cells. In the present study we describe isolation and characterization of LD from the industrial yeast Pichia pastoris. We designed and adapted an isolation procedure which allowed us to obtain this subcellular fraction at high purity as judged by quality control using appropriate marker proteins. Components of P. pastoris LD were characterized by conventional biochemical methods of lipid and protein analysis, but also by a lipidome and proteome approach. Our results show several distinct features of LD from P. pastoris especially in comparison to Saccharomyces cerevisiae. P. pastoris LD are characterized by their high preponderance of triacylglycerols over steryl esters in the core of the organelle, the high degree of fatty acid (poly)unsaturation and the high amount of ergosterol precursors. The high phosphatidylinositol to phosphatidylserine of ~7.5 ratio on the surface membrane of LD is noteworthy. Proteome analysis revealed equipment of the organelle with a small but typical set of proteins which includes enzymes of sterol biosynthesis, fatty acid activation, phosphatidic acid synthesis and non-polar lipid hydrolysis. These results are the basis for a better understanding of P. pastoris lipid metabolism and lipid storage and may be helpful for manipulating cell biological and/or biotechnological processes in this yeast.
- Find related publications in this database (using NLM MeSH Indexing)
- Base Sequence -
- DNA Primers -
- Electrophoresis, Polyacrylamide Gel -
- Lipids -
- Microscopy, Fluorescence -
- Pichia - metabolism
- Proteome -
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -
- Find related publications in this database (Keywords)
- Lipid droplet
- Triacylglycerol
- Steryl ester
- Lipidome
- Proteome
- Pichia pastoris