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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Khan, MJ; Alam, MR; Waldeck-Weiermair, M; Karsten, F; Groschner, L; Riederer, M; Hallstroem, S; Rockenfeller, P; Konya, V; Heinemann, A; Madeo, F; Graier, WF; Malli, R.
Inhibition of autophagy rescues palmitic acid induced necroptosis of endothelial cells.
J Biol Chem. 2012; 287(25):21110-21120
Doi: 10.1074/jbc.M111.319129
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- Führende Autor*innen der Med Uni Graz
- Khan Muhammad Jadoon
- Malli Roland
- Co-Autor*innen der Med Uni Graz
- Alam Muhammad Rizwan
- Graier Wolfgang
- Groschner Lukas
- Hallström Seth
- Heinemann Akos
- Karsten Felix Daniel
- Konya Viktoria
- Riederer Monika
- Waldeck-Weiermair Markus
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- Abstract:
- Accumulation of palmitic acid (PA) in cells from nonadipose tissues is known to induce lipotoxicity resulting in cellular dysfunction and death. The exact molecular pathways of PA-induced cell death are still mysterious. Here, we show that PA triggers autophagy, which did not counteract but in contrast promoted endothelial cell death. The PA-induced cell death was predominantly necrotic as indicated by annexin V and propidium iodide (PI) staining, absence of caspase activity, low levels of DNA hypoploidy, and an early ATP depletion. In addition PA induced a strong elevation of mRNA levels of ubiquitin carboxyl-terminal hydrolase (CYLD), a known mediator of necroptosis. Moreover, siRNA-mediated knockdown of CYLD significantly antagonized PA-induced necrosis of endothelial cells. In contrast, inhibition and knockdown of receptor interacting protein kinase 1 (RIPK1) had no effect on PA-induced necrosis, indicating the induction of a CYLD-dependent but RIPK1-independent cell death pathway. PA was recognized as a strong and early inducer of autophagy. The inhibition of autophagy by both pharmacological inhibitors and genetic knockdown of the autophagy-specific genes, vacuolar protein sorting 34 (VPS34), and autophagy-related protein 7 (ATG7), could rescue the PA-induced death of endothelial cells. Moreover, the initiation of autophagy and cell death by PA was reduced in endothelial cells loaded with the Ca(2+) chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-(acetoxymethyl) ester (BAPTA-AM), indicating that Ca(2+) triggers the fatal signaling of PA. In summary, we introduce an unexpected mechanism of lipotoxicity in endothelial cells and provide several novel strategies to counteract the lipotoxic signaling of PA.
- Find related publications in this database (using NLM MeSH Indexing)
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- Class III Phosphatidylinositol 3-Kinases - genetics Class III Phosphatidylinositol 3-Kinases - metabolism
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- RNA, Messenger - biosynthesis RNA, Messenger - genetics
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