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Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Dichlberger, A; Schlager, S; Lappalainen, J; Käkelä, R; Hattula, K; Butcher, SJ; Schneider, WJ; Kovanen, PT.
Lipid body formation during maturation of human mast cells.
J Lipid Res. 2011; 52(12):2198-2208
Doi: 10.1194/jlr.M019737
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- Co-Autor*innen der Med Uni Graz
- Schlager Stefanie
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- Abstract:
- Lipid droplets, also called lipid bodies (LB) in inflammatory cells, are important cytoplasmic organelles. However, little is known about the molecular characteristics and functions of LBs in human mast cells (MC). Here, we have analyzed the genesis and components of LBs during differentiation of human peripheral blood-derived CD34(+) progenitors into connective tissue-type MCs. In our serum-free culture system, the maturing MCs, derived from 18 different donors, invariably developed triacylglycerol (TG)-rich LBs. Not known heretofore, the MCs transcribe the genes for perilipins (PLIN)1-4, but not PLIN5, and PLIN2 and PLIN3 display different degrees of LB association. Upon MC activation and ensuing degranulation, the LBs were not cosecreted with the cytoplasmic secretory granules. Exogenous arachidonic acid (AA) enhanced LB genesis in Triacsin C-sensitive fashion, and it was found to be preferentially incorporated into the TGs of LBs. The large TG-associated pool of AA in LBs likely is a major precursor for eicosanoid production by MCs. In summary, we demonstrate that cultured human MCs derived from CD34(+) progenitors in peripheral blood provide a new tool to study regulatory mechanisms involving LB functions, with particular emphasis on AA metabolism, eicosanoid biosynthesis, and subsequent release of proinflammatory lipid mediators from these cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD34 - metabolism
- Arachidonic Acids - pharmacology
- Cell Degranulation - drug effects
- Cell Differentiation - drug effects
- Cytoplasm - drug effects Cytoplasm - metabolism
- Gene Expression Regulation - drug effects
- Humans -
- Lipid Metabolism - drug effects
- Lipids - chemistry
- Mast Cells - cytology Mast Cells - drug effects Mast Cells - metabolism
- Membrane Proteins - genetics
- Stem Cells - cytology Stem Cells - drug effects Stem Cells - metabolism
- Triglycerides - metabolism
- Vesicular Transport Proteins - genetics
- Find related publications in this database (Keywords)
- arachidonic acid
- eicosanoids
- fatty acid
- inflammation
- perilipin
- secretory granules
- triacylglycerol